Polymers of 2-methacryloyloxyethyl phosphorylcholine truly work as cell membrane mimic ?
Polymers of 2-methacryloyloxyethyl phosphorylcholine truly work as cell membrane mimic ?
复制标题
2-甲基丙烯酰氧基乙基磷酰胆碱聚合物确实可以作为细胞膜模拟物吗？
DOI：
10.1016/j.colsurfb.2010.12.033
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
and K. Hosoya
中科院分区：
文献类型：
--
作者：
T. Mori;T. Kubo;and K. Hosoya
我们制备了几种由2-甲基丙烯酰氧乙基磷酰胆碱（MPC）衍生的聚合物，以评估MPC聚合物是否作为细胞膜模拟物。首次应用对羧基苯磺酰胺（Sul）或布洛芬（Ibu）作为探针对靶蛋白进行捕获实验。结果表明，基于MPC的亲水性聚合物能够抑制非特异性结合蛋白。此外，当Sul用作探针时，一些基于MPC的聚合物表面能够捕获比其他聚合物更大量的碳酸酐酶II。相反，所有具有PC基团和Ibu探针的聚合物都不能捕获其靶蛋白环氧合酶-1（考克斯-1）。这些结果表明，PC基团的位置实现了聚合物表面的亲水性，而MPC基聚合物不能为考克斯-1与Ibu的相互作用提供合适的环境。
We have prepared several types of polymers derived from 2-methacryloyloxyethyl phosphorylcholine (MPC) to evaluate whether polymers of MPC work as cell membrane mimic or not. We firstly applied capturing test of target proteins of 4-carboxybenzenesulfonamide (Sul) or ibuprofen (Ibu) as a probe. As the results, the rather hydrophilic polymers based on MPC were able to suppress non-specific binding proteins as expected. Additionally, some of the MPC based polymeric surface was able to capture greater amount of carbonic anhydrase II than those of other polymers, when Sul was utilized as probe. In contrary, all the polymers having PC groups and Ibu probe were unable to capture Cyclooxygenase-1 (COX-1), its target protein. These results suggested that the position of PC groups realized hydrophilic polymer surface, while MPC based polymer was not able to supply the suitable environment for COX-1 to interact with Ibu.