[Effect of Atractylodes macrocephala polysaccharide on proliferation and invasion of hepatocellular carcinoma cells in vitro].
[Effect of Atractylodes macrocephala polysaccharide on proliferation and invasion of hepatocellular carcinoma cells in vitro].
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DOI：
10.12122/j.issn.1673-4254.2019.10.08
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发表时间：
2019-10
影响因子：
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通讯作者：
Yun Zhu;Cheng Li;Xinsheng Lin;Jingjing Sun;Yang Cheng
中科院分区：
文献类型：
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作者：
Yun Zhu;Cheng Li;Xinsheng Lin;Jingjing Sun;Yang Cheng
目的探讨苍术多糖对肝癌细胞增殖和侵袭的抑制作用及其机制。方法用不同浓度的PAM处理肝癌HepG2细胞，采用CCK-8法和Transwell法检测其增殖和侵袭能力。免疫荧光法检测细胞中β-catenin的表达水平，real-time PCR和Western blotting检测细胞中AKT、GSK-3β和MMP-2 mRNA和蛋白的表达。观察LiCl/PAM/LiCl + PAM处理后细胞增殖、侵袭性及pGSK-3β、MMP2表达的变化。结果PAM处理显著降低细胞活力、迁移细胞数量、β-catenin和MMP-2表达水平（P < 0.05），抑制细胞中AKT和GSK-3β磷酸化（P < 0.05），并呈剂量依赖性。拯救实验表明，LiCl逆转了PAM诱导的细胞增殖、侵袭性和Wnt/β-catenin通路的抑制作用。结论PAM可能通过抑制Wnt/β-catenin信号通路，在体外抑制肝癌细胞的增殖和侵袭。
OBJECTIVE To investigate the inhibitory effect of polysaccharide of Atractylodes macrocephala (PAM) on the proliferation and invasion of hepatocellular carcinoma cells and the underlying mechanism. METHODS Hepatocellular carcinoma HepG2 cells were treated with different concentrations of PAM, and their proliferation and invasive ability were examined using CCK-8 assay and Transwell assay. Immunofluorescence assay was performed to detect the expression level of β-catenin, and real-time PCR and Western blotting were used to detect the mRNA and protein expressions of AKT, GSK-3β and MMP-2 in the cells. The changes in the proliferation, invasiveness and the expressions of pGSK-3β and MMP2 were examined in the cells following treatment with LiCl/PAM/LiCl plus PAM. RESULTS PAM treatment significantly reduced the cell viability, the number of migration cells, and the expression levels of β-catenin and MMP-2 (P < 0.05), and obviously inhibited the phosphorylation of AKT and GSK-3β in the cells (P < 0.05) in a dose-dependent manner. The rescue experiment showed that LiCl reversed the inhibition of cell proliferation, invasiveness, and the Wnt/β-catenin pathway induced by PAM. CONCLUSIONS PAM can inhibit the proliferation and invasion of hepatocellular carcinoma cells in vitro possibly by inhibiting the Wnt/β-catenin signaling pathway.