HEAD-GROUP CONFORMATION IN PHOSPHOLIPIDS - P-31 NUCLEAR MAGNETIC-RESONANCE STUDY OF ORIENTED MONODOMAIN DIPALMITOYLPHOSPHATIDYLCHOLINE BILAYERS
HEAD-GROUP CONFORMATION IN PHOSPHOLIPIDS - P-31 NUCLEAR MAGNETIC-RESONANCE STUDY OF ORIENTED MONODOMAIN DIPALMITOYLPHOSPHATIDYLCHOLINE BILAYERS
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DOI：
10.1021/bi00607a004
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发表时间：
1978-01-01
期刊：
影响因子：
2.9
通讯作者：
PERSHAN, PS
中科院分区：
文献类型：
--
作者：
GRIFFIN, RG;POWERS, L;PERSHAN, PS
取向双轴单畴DPPC的角度依赖性31P NMR光谱采用H2O多层膜研究了该磷脂中的头基构象。磷酸盐的O-P-O平面（其中O是磷酸二酯的非酯化氧）倾斜47 ° ±。5.degree.相对于双层法线。这种PO4取向可能导致胆碱部分平行于双层平面延伸，这将解释DPPC在过量水中观察到的轴对称31P粉末光谱的宽度。这项工作是第一次直接观察到这种构象的卵磷脂，它说明了高分辨率的固体核磁共振在无序系统的结构研究的效用。
Angular-dependent 31P NMR spectra of oriented biaxial monodomain DPPC .cntdot. H2O multilayers are employed to study head-group conformation in this phospholipid. The O-P-O plane of the phosphate, where the O are the nonesterified oxygens of the phosphodiester, is tilted at 47 .+-. 5.degree. with respect to the bilayer normal. This PO4 orientation could result in the choline moiety being extended parallel to the bilayer plane, and it will explain the breadth of the axially symmetric 31P powder spectrum observed for DPPC in excess water. This work is the 1st direct observation of this conformation for lecithins and it illustrates the utility of high-resolution solid-state NMR in structural studies of disordered systems.