Mutagenesis of murine granulocyte/macrophage-colony-stimulating factor reveals critical residues near the N terminus.
Mutagenesis of murine granulocyte/macrophage-colony-stimulating factor reveals critical residues near the N terminus.
复制标题
小鼠粒细胞/巨噬细胞集落刺激因子的诱变揭示了 N 末端附近的关键残基。
DOI：
10.1111/j.1432-1033.1987.tb13619.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Metcalf,D
中科院分区：
文献类型：
--
作者：
Gough,NM;Grail,D;Gearing,DP;Metcalf,D
A number of cDNAs encoding mutant forms of the murine haemopoietic growth factor, granulocyte/macrophage‐colony‐stimulating factor (GM‐CSF), have been derived byin vitromutagenesis and expressed in simian COS cells. Determination of the biological activity of the mutant factors revealed that residues within the regions 11–15, 24–37, 47–49 and 81–89 are required for generating a functional GM‐CSF molecule. In particular, truncation of either of two strongly predicted α helices near the N terminus of the molecule severely depresses the activity of the factor.
