Transgenic zebrafish reveal stage-specific roles for Bmp signaling in ventral and posterior mesoderm development
Transgenic zebrafish reveal stage-specific roles for Bmp signaling in ventral and posterior mesoderm development
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DOI：
10.1242/dev.01806
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发表时间：
2005-05-01
期刊：
影响因子：
4.6
通讯作者：
Kimelman, D
中科院分区：
文献类型：
--
作者：
Pyati, UJ;Webb, AE;Kimelman, D
骨形态发生蛋白（Bmp）信号对斑马鱼腹侧和后中胚层的形成和图案化至关重要。Bmp通路缺陷的突变体具有膨胀的躯干肌肉、异常的尾部和腹侧中胚层衍生物（例如脉管系统、血液和原肾）的严重受损的发育。由于BMPs在原肠胚形成后继续在腹侧和后中胚层中表达，因此BMPs信号传导可能在后体的生长过程中继续发挥重要的发育作用。然而，由于Bmp信号在原肠胚阶段起着至关重要的作用，因此不可能用突变体或标准破坏技术来确定Bmp途径的后期功能。为了研究Bmp信号传导在躯干和尾部生长过程中腹侧和后部中胚层中的作用，我们产生了含有热休克诱导的显性负性Bmp受体-GFP融合的转基因斑马鱼系。我们的数据表明，BMPs是重要的尾部组织者的形成和图案的腹侧中胚层在早期原肠胚。然而，从中期原肠胚到早期体节形成阶段，BMP信号是重要的腹尾鳍的发展和防止次生尾的形成。我们得出结论，BMP信号在腹侧和后中胚层的作用的变化，原肠胚的进行。
Bone morphogenetic protein (Bmp) signaling is crucial for the formation and patterning of zebrafish ventral and posterior mesoderm. Mutants defective in the Bmp pathway have expanded trunk muscle, abnormal tails and severely impaired development of ventral mesodermal derivatives such as vasculature, blood and pronephros. As Bmps continue to be expressed in the ventral and posterior mesoderm after gastrulation, it is likely that Bmp signaling continues to play an important developmental role during outgrowth of the posterior body. However, because Bmp signaling plays an essential role during the gastrula stages, it has not been possible with mutants or standard disruption techniques to determine the later functions of the Bmp pathway. To study the role of Bmp signaling in the ventral and posterior mesoderm during trunk and tail outgrowth, we generated a transgenic zebrafish line containing a heatshock-inducible dominant-negative Bmp receptor-GFP fusion. Our data show that Bmps are important for tail organizer formation and for patterning the ventral mesoderm during early gastrulation. However, from mid-gastrulation to the early somitogenesis stages, Bmp signaling is important for ventral tail fin development and for preventing secondary tail formation. We conclude that the role of Bmp signaling in the ventral and posterior mesoderm changes as gastrulation proceeds.