Cloning and Expression Analysis of the Soybean CO-Like Gene GmCOL9
Cloning and Expression Analysis of the Soybean CO-Like Gene GmCOL9
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DOI：
10.1007/s11105-010-0240-y
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发表时间：
2011-06-01
影响因子：
2.1
通讯作者：
Fu, Yong-Fu
中科院分区：
文献类型：
--
作者：
Huang, Guowen;Ma, Jinghua;Fu, Yong-Fu
光周期是开花的重要环境因子之一，在模式植物拟南芥中，Constans(CO)基因是这一途径的关键调控基因。在许多植物物种中都存在类似辅酶的基因。本文描述了从大豆品种科农18中分离到的CO同源基因Glyine max Constans-like 9(GmCOL9)。序列比较表明，与GmCOL9最接近的拟南芥基因是COL13。用实时定量逆转录聚合酶链式反应研究了GmCOL9的表达，结果表明GmCOL9的表达不受昼夜节律机制的调控，其表达周期为12h，而不是24h，GmCOL9的表达受植物发育阶段的影响，并在组织/器官之间存在差异。GmCOL9的表达主要集中在营养生长期的单叶叶片和生殖生长期的茎中。然而，GmCOL9在整个植株中也有表达的痕迹，这表明GmCOL9可能是大豆植株发育过程中的一个多功能基因。
Photoperiod is one of the important environmental triggers of flowering, and the CONSTANS (CO) gene is a key regulator of this pathway in the model plant Arabidopsis thaliana. CO-like genes are present in many plant species. We describe here the isolation of the CO homolog Glycine max CONSTANS-like 9 (GmCOL9) from the soybean cultivar Kennong18. Sequence comparisons showed that the closest A. thaliana gene to GmCOL9 is COL13. The expression of GmCOL9 was investigated using quantitative real-time reverse transcriptase polymerase chain reaction, which showed that GmCOL9 was not regulated by a circadian mechanism, since its expression oscillated at a cycle of 12 h instead of 24 h. GmCOL9 expression was influenced by the developmental stage of the plant and varied between tissue/organs. The expression of GmCOL9 was concentrated in the unifoliolate leaves during the vegetative phase of growth and in the stem during the reproductive phase. However, traces of expression were also present throughout the plant, indicating that GmCOL9 may be a multifunctional gene in the context of the development of the soybean plant.