PARTHENOGENETIC ACTIVATION OF HUMAN OOCYTES FOLLOWING CRYOPRESERVATION USING 1,2-PROPANEDIOL
PARTHENOGENETIC ACTIVATION OF HUMAN OOCYTES FOLLOWING CRYOPRESERVATION USING 1,2-PROPANEDIOL
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DOI：
10.1093/oxfordjournals.humrep.a136005
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发表时间：
1995-03-01
影响因子：
6.1
通讯作者：
JOHNSTON, WIH
中科院分区：
文献类型：
--
作者：
GOOK, DA;OSBORN, SM;JOHNSTON, WIH
新鲜和老化的人卵母细胞冷冻保存使用1，2-丙二醇（PROH）。解冻后，将卵母细胞培养20 h，并使用光学显微镜和紫外DNA染色检查孤雌激活。还检查了对照新鲜或老化卵母细胞和暴露于PROH而未冷冻保存的卵母细胞的激活。未观察到对照卵母细胞自发激活（n = 43），单独暴露于PROH也未诱导孤雌激活（n = 26）。在新鲜和老化的冻存卵母细胞中，分别有27%和29%的卵母细胞被激活，这些比例与对照组相比显著升高（P < 0.01），新鲜卵母细胞和老化卵母细胞冻存后的孤雌激活率相近，但激活形式不同，在18%的新鲜卵母细胞和5%的老化冻存卵母细胞中观察到单个原核。与此相反，两个或两个以上的原核的存在下，观察到在0%的新鲜和19%的老年冻存卵母细胞。
Fresh and aged human oocytes were cryopreserved using 1,2-propanediol (PROH). After thawing, the oocytes were cultured for 20 h and examined for parthenogenetic activation using light microscopy and an ultraviolet DNA stain, Control fresh or aged oocytes and oocytes exposed to PROH without cryopreservation were also examined for activation, No control oocytes were observed to activate spontaneously (n = 43) and parthenogenetic activation was not induced by exposure to PROH alone (n = 26), In both fresh and aged cryopreserved oocytes, 27 and 29% of the oocytes respectively were activated, and these proportions were significantly elevated compared with the controls (P < 0.01), Although a similar rate of activation was observed for the cryopreserved fresh and aged oocytes, the form of parthenogenetic activation varied between these two types of oocyte, A single pronucleus was observed in 18% of the fresh and 5% of the aged cryopreserved oocytes. In contrast, the presence of two or more pronuclei was observed in 0% of the fresh and 19% of the aged cryopreserved oocytes.