Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative.
Purkinje Cardiomyocytes of the Adult Ventricular Conduction System Are Highly Diploid but Not Uniquely Regenerative.
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DOI：
10.3390/jcdd10040161
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发表时间：
2023-04-07
影响因子：
2.4
通讯作者：
中科院分区：
文献类型：
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作者：
成人心脏的特点是损伤后再生效率低下，因此，支持或防止心肌细胞（CM）增殖的功能是重要的澄清。二倍体CM是一种可能具有独特增殖和再生能力的候选细胞类型，但目前还没有已知的分子标记物可以选择性地鉴定二倍体CM的所有或亚群。在这里，使用传导系统表达标记Cntn 2-GFP和传导系统谱系标记Etv 1CreERT 2，我们证明了包括成人心室传导系统的浦肯野CM是不成比例的二倍体（33%，相对于体积心室CM的4%）。然而，这些仅占总二倍体CM群体的一小部分（3%）。使用EdU掺入在出生后第一周，我们证明了散装二倍体CM发现在后来的心脏进入并完成细胞周期在新生儿时期。与此相反，一个显着的分数传导CM坚持作为二倍体细胞从胎儿的生活，避免新生儿细胞周期的活动。尽管他们的高度二倍体，浦肯野血统没有增强能力，以支持成人心肌梗死后的再生。
Adult hearts are characterized by inefficient regeneration after injury, thus, the features that support or prevent cardiomyocyte (CM) proliferation are important to clarify. Diploid CMs are a candidate cell type that may have unique proliferative and regenerative competence, but no molecular markers are yet known that selectively identify all or subpopulations of diploid CMs. Here, using the conduction system expression marker Cntn2-GFP and the conduction system lineage marker Etv1CreERT2, we demonstrate that Purkinje CMs that comprise the adult ventricular conduction system are disproportionately diploid (33%, vs. 4% of bulk ventricular CMs). These, however, represent only a small proportion (3%) of the total diploid CM population. Using EdU incorporation during the first postnatal week, we demonstrate that bulk diploid CMs found in the later heart enter and complete the cell cycle during the neonatal period. In contrast, a significant fraction of conduction CMs persist as diploid cells from fetal life and avoid neonatal cell cycle activity. Despite their high degree of diploidy, the Purkinje lineage had no enhanced competence to support regeneration after adult heart infarction.
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影响因子：
2.5
作者：
Omatsu-Kanbe M;Yoshioka K;Fukunaga R;Sagawa H;Matsuura H
通讯作者：
Matsuura H
影响因子：
11.8
作者：
Gan, Peiheng;Wang, Zhaoning;Morales, Maria Gabriela;Zhang, Yu;Bassel-Duby, Rhonda;Liu, Ning;Olson, Eric N.
通讯作者：
Olson, Eric N.
Sema3a-CreERT2 对浦肯野纤维进行遗传靶向
影响因子：
4.6
作者：
Li Y;Tian X;Zhao H;He L;Zhang S;Huang X;Zhang H;Miquerol L;Zhou B
通讯作者：
Zhou B
DOI：
10.1161/circep.109.928820
发表时间：
2010-04
期刊：
Circulation. Arrhythmia and electrophysiology
影响因子：
--
作者：
Pallante BA;Giovannone S;Fang-Yu L;Zhang J;Liu N;Kang G;Dun W;Boyden PA;Fishman GI
通讯作者：
Fishman GI
影响因子：
11.8
作者：
Han, Lu;Choudhury, Sangita;Kuhn, Bernhard
通讯作者：
Kuhn, Bernhard