NEW HUMAN TRANSFORMING GENES DETECTED BY A TUMORIGENICITY ASSAY
NEW HUMAN TRANSFORMING GENES DETECTED BY A TUMORIGENICITY ASSAY
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DOI：
10.1128/mcb.4.9.1695
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发表时间：
1984-01-01
影响因子：
5.3
通讯作者：
WIGLER, M
中科院分区：
文献类型：
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作者：
FASANO, O;BIRNBAUM, D;WIGLER, M
基于NIH3T3细胞在裸鼠体内的致瘤性，建立了一种灵敏的转化基因的生物检测方法。该检测方法与NIH3T3焦点检测方法有很大不同。用该方法检测了人乳腺癌细胞株MCF-7 DNA中3个转化基因的转移。其中之一是N-ras，它在MCF-7 DNA中被扩增。另外两个被称为mcf2和mcf3的基因似乎与已知的癌基因无关。用NIH3T3焦点法无法检测到它们的转移。MCF2或MCF3是否与MCF-7细胞的遗传异常有关尚不清楚。
A sensitive bioassay for transforming genes based on the tumorigenicity of cotransfected NIH3T3 cells in nude mice was developed. The assay differs substantially from the NIH3T3 focus assay. Using it, the transfer of 3 transforming genes from the DNA of MCF-7, a human mammary carcinoma cell line was detected. One of these is N-ras, which is amplified in MCF-7 DNA. The other two, which were called mcf2 and mcf3, do not appear to be related to known oncogenes. Their transfer could not be detected by using the NIH3T3 focus assay. Whether either mcf2 or mcf3 is associated with genetic abnormalities in MCF-7 cells is not yet known.