The Sugar Transporter Inventory of Tomato: Genome-Wide Identification and Expression Analysis
The Sugar Transporter Inventory of Tomato: Genome-Wide Identification and Expression Analysis
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DOI：
10.1093/pcp/pcu052
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发表时间：
2014-06-01
影响因子：
4.9
通讯作者：
Shiratake, Katsuhiro
中科院分区：
文献类型：
--
作者：
Reuscher, Stefan;Akiyama, Masahito;Shiratake, Katsuhiro
糖在植物源组织和库组织之间的流动依赖于糖转运蛋白。研究相应的基因可以操纵正在发育的水果的库强度，从而提高供人类食用的水果质量。番茄（Solanum lycopersicum）是一种重要的园艺作物，也是肉质果实发育的典范。在这篇文章中，我们提供了一个全面的清单番茄糖转运蛋白，包括糖转运蛋白家族，糖转运蛋白蛋白家族，糖促进蛋白家族，多元醇/单糖转运蛋白家族，肌醇转运蛋白家族，塑料葡萄糖转运蛋白家族，液泡膜单糖转运蛋白家族和真空葡萄糖转运蛋白家族。表达序列标签（EST）测序和系统发育分析建立了所有分析的番茄糖转运蛋白的命名。我们总共鉴定了52个番茄红素编码糖转运蛋白的基因。分析了29个糖转运蛋白基因在营养组织和果实发育过程中的表达。几个糖转运蛋白基因表达的组织或发育阶段的特定方式。这些信息将有助于更好地了解番茄光合物质的源库运动。鉴定果实特异性糖转运蛋白可能是寻找番茄果实糖积累相关基因的第一步。
The mobility of sugars between source and sink tissues in plants depends on sugar transport proteins. Studying the corresponding genes allows the manipulation of the sink strength of developing fruits, thereby improving fruit quality for human consumption. Tomato (Solanum lycopersicum) is both a major horticultural crop and a model for the development of fleshy fruits. In this article we provide a comprehensive inventory of tomato sugar transporters, including the SUCROSE TRANSPORTER family, the SUGAR TRANSPORTER PROTEIN family, the SUGAR FACILITATOR PROTEIN family, the POLYOL/MONOSACCHARIDE TRANSPORTER family, the INOSITOL TRANSPORTER family, the PLASTIDIC GLUCOSE TRANSLOCATOR family, the TONOPLAST MONOSACCHARIDE TRANSPORTER family and the VACUOLAR GLUCOSE TRANSPORTER family. Expressed sequence tag (EST) sequencing and phylogenetic analyses established a nomenclature for all analyzed tomato sugar transporters. In total we identified 52 genes in tomato putatively encoding sugar transporters. The expression of 29 sugar transporter genes in vegetative tissues and during fruit development was analyzed. Several sugar transporter genes were expressed in a tissue- or developmental stage-specific manner. This information will be helpful to better understand source to sink movement of photoassimilates in tomato. Identification of fruit-specific sugar transporters might be a first step to find novel genes contributing to tomato fruit sugar accumulation.