Hot-nanoparticle-mediated fusion of selected cells
Hot-nanoparticle-mediated fusion of selected cells
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DOI：
10.1007/s12274-016-1392-3
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发表时间：
2017-06-01
期刊：
影响因子：
9.9
通讯作者：
Bendix, Poul M.
中科院分区：
文献类型：
--
作者：
Bahadori, Azra;Oddershede, Lene B.;Bendix, Poul M.
两个选择的细胞的完全融合允许产生具有来自两个原始细胞的遗传特性的新型杂交细胞。或者，通过所选细胞与所选囊泡的融合，可以将化学品或基因直接递送到感兴趣的细胞中，以控制细胞反应或基因表达。在这里，我们演示了如何进行两个选定的细胞或一个细胞和一个囊泡的光学控制融合。融合是通过激光照射等离子体金纳米粒子光学捕获之间的两个细胞（或囊泡和细胞）的利益。这种热颗粒介导的融合导致两种细胞因子和两种细胞膜的完全混合，导致融合后形成具有完整细胞膜和酶活性的新杂交细胞。类似地，囊泡和细胞之间的融合导致囊泡货物递送至细胞质，并且在融合后，细胞显示出存活的迹象。该方法是在单细胞水平上实现靶向药物递送的方法，并且在细胞控制和设计方面具有巨大的潜力。
Complete fusion of two selected cells allows for the creation of novel hybrid cells with inherited genetic properties from both original cells. Alternatively, via fusion of a selected cell with a selected vesicle, chemicals or genes can be directly delivered into the cell of interest, to control cellular reactions or gene expression. Here, we demonstrate how to perform an optically controlled fusion of two selected cells or of one cell and one vesicle. Fusion is mediated by laser irradiating plasmonic gold nanoparticles optically trapped between two cells (or a vesicle and a cell) of interest. This hot-particle-mediated fusion causes total mixing of the two cytoplasms and the two cell membranes resulting in formation of a new hybrid cell with an intact cell membrane and enzymatic activity following fusion. Similarly, fusion between a vesicle and a cell results in delivery of the vesicle cargo to the cytoplasm, and after fusion, the cell shows signs of viability. The method is an implementation of targeted drug delivery at the single-cell level and has a great potential for cellular control and design.