Antibacterial efficacy of cold atmospheric plasma against Enterococcus faecalis planktonic cultures and biofilms in vitro
Antibacterial efficacy of cold atmospheric plasma against Enterococcus faecalis planktonic cultures and biofilms in vitro
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DOI：
10.1371/journal.pone.0223925
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发表时间：
2019-11
期刊：
影响因子：
3.7
通讯作者：
Felix Theinkom;Larissa Singer;F. Cieplik;Sylvia Cantzler;Hannes Weilemann;Maximilian Cantzler;K. H
中科院分区：
文献类型：
--
作者：
Felix Theinkom;Larissa Singer;F. Cieplik;Sylvia Cantzler;Hannes Weilemann;Maximilian Cantzler;K. H
在我们这个时代，医院感染已经成为一个严重的威胁，并且由于细菌耐药性的增加而变得越来越难以处理。在这种情况下，冷大气等离子体（CAP），这是已知的有效地杀灭微生物，可能是一个有前途的替代应用在牙科和皮肤科领域。CAP是部分电离的气体，其在低温下操作，并且由电子、离子、受激原子和分子、活性氧和氮物质组成。在这项研究中，从环境空气中产生的CAP对粪肠球菌的影响进行了研究，在琼脂平板上生长或作为生物膜培养长达72小时。CAP使琼脂平板上的殖民地形成单位（CFU）减少> 7 log10步。处理24 h的E.粪球菌导致CFU减少≥ 3 log10步骤后，CAP治疗5分钟，并由≥ 5 log10步骤后，CAP治疗10分钟。在生物膜实验中，洗必泰（CHX）和UVC辐射作为阳性对照，仅略高于CAP的有效性。如核酸释放的光谱测量所示，CAP处理后细胞质膜没有损伤。因此，膜损伤似乎不是CAP对大肠杆菌的主要作用机制。粪便总的来说，CAP对大肠杆菌显示出明显的抗菌效果。在琼脂平板上以及在生物膜中，类似于阳性对照CHX或UVC。
Nosocomial infections have become a serious threat in our times and are getting more difficult to handle due to increasing development of resistances in bacteria. In this light, cold atmospheric plasma (CAP), which is known to effectively inactivate microorganisms, may be a promising alternative for application in the fields of dentistry and dermatology. CAPs are partly ionised gases, which operate at low temperature and are composed of electrons, ions, excited atoms and molecules, reactive oxygen and nitrogen species. In this study, the effect of CAP generated from ambient air was investigated against Enterococcus faecalis, grown on agar plates or as biofilms cultured for up to 72 h. CAP reduced the colony forming units (CFU) on agar plates by > 7 log10 steps. Treatment of 24 h old biofilms of E. faecalis resulted in CFU-reductions by ≥ 3 log10 steps after CAP treatment for 5 min and by ≥ 5 log10 steps after CAP treatment for 10 min. In biofilm experiments, chlorhexidine (CHX) and UVC radiation served as positive controls and were only slightly more effective than CAP. There was no damage of cytoplasmic membranes upon CAP treatment as shown by spectrometric measurements for release of nucleic acids. Thus, membrane damage seems not to be the primary mechanism of action for CAP towards E. faecalis. Overall, CAP showed pronounced antimicrobial efficacy against E. faecalis on agar plates as well as in biofilms similar to positive controls CHX or UVC.