EFFECT OF ORAL TETRACYCLINE, THE MICROBIAL-FLORA, AND THE ATHYMIC STATE ON GASTRO-INTESTINAL COLONIZATION AND INFECTION OF BALB-C MICE WITH CANDIDA-ALBICANS
EFFECT OF ORAL TETRACYCLINE, THE MICROBIAL-FLORA, AND THE ATHYMIC STATE ON GASTRO-INTESTINAL COLONIZATION AND INFECTION OF BALB-C MICE WITH CANDIDA-ALBICANS
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DOI：
10.1128/iai.23.3.764-774.1979
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
BALISH, E
中科院分区：
文献类型：
--
作者：
HELSTROM, PB;BALISH, E
采用扫描电镜、光学显微镜和肠道内容物微生物定量培养等方法，研究口服免疫和胸腺依赖性免疫能力对C.白色念珠菌定殖并感染4组小鼠的胃肠道：胸腺完整的常规小鼠、常规无胸腺小鼠、菌群确定的无胸腺小鼠和胸腺完整的无菌小鼠。胸腺完整的常规小鼠在没有抗生素治疗的情况下开始脱落C。在口服酵母菌攻击后48小时内检测到白色念珠菌，并且到第16天没有可检测到的酵母菌。四环素改变了细菌植物群的性质和数量，使C.白色念珠菌在不到48小时内定殖，并在肠道中持续32天。72只这些常规小鼠中只有2只发生念珠菌病（菌丝感染）。虽然四环素改变了传统无胸腺（裸）小鼠的细菌植物群，但不需要让C.白色念珠菌定殖其肠道的水平显著高于胸腺完整的常规小鼠。所有传统的裸鼠一致定殖和14的24只动物表现出增加的酵母菌定殖的角化胃，但只有3的24发展胃念珠菌病。弗洛拉定义的无胸腺（裸）小鼠有显着较低的需氧菌水平和显着较高的C。白色念珠菌水平的肠道内容物比传统的无胸腺小鼠。菌群确定的裸鼠在第5天发生胃念珠菌病。胸腺完整的无菌小鼠均匀定殖并感染C。经口激发后48 h内出现白色念珠菌，无论是否接受四环素治疗。C的人口。无菌小鼠肠道中的白色念珠菌显著高于胸腺完整的常规或无胸腺小鼠肠道中的白色念珠菌。经口攻毒后2天，10只无菌动物中有8只检出胃菌丝体感染。到第32天，四环素治疗组和对照无菌组的50只小鼠中有45只感染了C。白色念珠菌在预防胃念珠菌病方面，竞争性细菌植物群可能比完整的免疫系统更有效，并且免疫缺陷可能使角化和腺胃上皮的酵母定殖增加。四环素似乎没有增强C.尽管它促进了常规饲养小鼠中酵母相肠道定殖，但它在小鼠中仍然抑制了白色念珠菌。
Scanning electron microscopy, light microscopy and quantitative culture of microorganisms in intestinal contents were used to determine the effects of oral and thymus-dependent immune competency on the capacity of C. albicans to colonize and infect the gastrointestinal tract of 4 groups of mice: thymus-intact conventional mice, conventional athymic mice, flora-defined athymic mice, and thymus-intact bacteria-free mice. Thymus-intact conventional mice without antibiotic treatment began to shed C. albicans less than 48 h after oral yeast challenge and were devoid of detectable yeast by day 16. Tetracycline altered the bacterial flora qualitatively and quantitatively, allowing C. albicans to colonize in less than 48 h and to persist in the gut tract for 32 days. Only 2 of 72 of these conventional mice developed candidiasis (hyphal infection). Although tetracycline altered the bacterial flora of conventional athymic (nude) mice, it was not required to allow C. albicans to colonize their gut tract to levels significantly higher than those in thymus-intact conventional mice. All conventional nude mice were consistently colonized and 14 of 24 animals showed an increased yeast colonization of the keratinized stomach, but only 3 of 24 developed gastric candidiasis. Flora-defined athymic (nude) mice had significantly lower aerobic bacterial levels and significantly higher C. albicans levels in the gut contents than conventional athymic mice. The flora-defined nude mice developed gastric candidiasis by day 5. Thymus-intact bacteria-free mice were uniformly colonized and infected with C. albicans less than 48 h after oral challenge regardless of tetracycline treatment. Populations of C. albicans in the gut of bacteria-free mice were significantly higher than in the gut tract of the thymus-intact conventional or athymic mice. Gastric mycelial infection was detected in 8 of 10 bacteria-free animals 2 days after oral challenge. By 32 days, 45 of 50 mice of both tetracycline-treated and control bacteria-free groups were infected with C. albicans. A competitive bacteria flora may be more effective than an intact immune system in preventing gastric candidiasis and an immune deficiency may allow increased yeast colonization of the keratinized and glandular stomach epithelium. Tetracycline did not appear to enhance the invasiveness or pathogenicity of C. albicans in mice even though it facilitates yeast-phase gut colonization in conventionally reared mice.