Neonatal release of gonadotropins is essential for development of ovarian follicle-stimulating hormone receptors.
Neonatal release of gonadotropins is essential for development of ovarian follicle-stimulating hormone receptors.
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新生儿促性腺激素的释放对于卵泡刺激激素受体的发育至关重要。
DOI：
10.1095/biolreprod34.1.219
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发表时间：
1986
影响因子：
3.6
通讯作者：
Ojeda,SR
中科院分区：
文献类型：
--
作者：
Smith,SS;Ojeda,SR
在大鼠中，卵巢卵泡刺激素（FSH）受体在出生后的前两周显著增加，此时血清促性腺激素水平最高。本研究旨在评估以下假设：这些高促性腺激素水平，特别是FSH，参与发育中卵巢获得FSH受体。促性腺激素的释放被抑制的几个非芳香化雄激素，其中丙酸二氢睾酮（DHTP）是最有效的管理。在一系列实验中，从第5天至第11天给予类固醇，并在第12天测量血清FSH和黄体生成素（LH）。令人惊讶的是，FSH受体含量在血清促性腺激素抑制的大鼠中高于对照组。在DHTP处理的大鼠中观察到可用受体的最大增加，其中血清FSH降低至对照值的20%，LH抑制至不可检测值。DHTP不能直接增加垂体切除的未成年大鼠的可用FSH受体。氯化镁（MgCl 2）处理卵巢膜去除结合125 I-hFSH的87%，而不影响受体活力。对照组12日龄卵巢暴露于MgCl 2可使可用FSH受体增加至与未暴露于MgCl 2的DHTP处理大鼠卵巢相似的水平，表明DHTP处理大鼠中可用更多受体，因为血清FSH受到抑制。早期开始DHTP治疗（出生后第1天）抑制血清FSH和LH至第5天检测不到的值，并在第12天将FSH受体含量降低至对照值以下。MgCl 2处理仅略微增加了这些DTTP处理动物的可用受体。早期DHTP治疗对FSH受体的抑制作用被新生儿（第1天至第5天）施用纯化的人FSH，表明受体含量的下降是血清FSH水平降低的结果，而不是由于DHTP对卵巢的直接作用。在新生期，对于随后卵巢FSH受体的出现是必需的，和B）在婴儿期，血清FSH滴度小于对照值的五分之一，但与幼年大鼠中存在的水平相似，足以将卵巢FSH受体含量维持在正常水平，尽管检测不到血清LH水平。
In the rat, ovarian follicle-stimulating hormone (FSH) receptors increase markedly during the first two postnatal weeks, when serum gonadotropin levels are most elevated. This study was conducted to evaluate the hypothesis that these high gonadotropin levels, and in particular FSH, are involved in the acquisition of FSH receptors by the developing ovary. Gonadotropin release was suppressed by administration of several non-aromatizable androgens, among which dihydrotestosterone propionate (DHTP) was the most effective. In one series of experiments the steroids were administered from Days 5 to 11, and serum FSH and luteinizing hormone (LH) were measured on Day 12. Surprisingly, FSH receptor content was greater in rats with suppressed serum gonadotropins than in controls. The greatest increase in available receptors was observed in DHTP-treated rats in which serum FSH was reduced to 20% of control values and LH suppressed to undetectable values. DHTP failed to directly increase available FSH receptors in hypophysectomized immature rats. Magnesium chloride (MgCl2) treatment of ovarian membranes removed bound125I-hFSH by 87% without affecting receptor viability. Exposure of control 12-day-old ovaries to MgCl2increased available FSH receptors to a level similar to that of ovaries from DHTP-treated rats not exposed to MgCl2, suggesting that more receptors were available in DHTP-treated rats because serum FSH was suppressed. Earlier initation of DHTP treatment (postnatal Day 1) suppressed serum FSH and LH to undetectable values by Day 5 and decreased FSH receptor content below control values by Day 12. MgCl2treatment only slightly increased available receptors in these DHTP-treated animals. The suppressive effect of early DHTP treatment on FSH receptors was prevented by neonatal (Days I to 5) administration of purified human FSH, suggesting that the decline in receptor content was a consequence of depressed serum FSH levels, and not due to a direct DHTP action on the ovary.It is concluded that a) elevated levels of serum gonadotropins, particularly FSH, during neonatal days are essential for the subsequent appearance of ovarian FSH receptors, and b) during the infantile period, serum FSH titers less than one-fifth of control values, but similar to levels present in juvenile rats, suffice to maintain ovarian FSH receptor content at normal levels, in spite of undetectable levels of serum LH.