RecA protein promotes rapid RNA-DNA hybridization in heterogeneous RNA mixtures.
RecA protein promotes rapid RNA-DNA hybridization in heterogeneous RNA mixtures.
复制标题
RecA 蛋白促进异质 RNA 混合物中的快速 RNA-DNA 杂交。
DOI：
10.1093/nar/20.16.4347
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发表时间：
1992
影响因子：
14.9
通讯作者：
Radding,CM
中科院分区：
文献类型：
--
作者：
Kirkpatrick,DP;Radding,CM
Escherichla coll的RecA蛋白在单链DNA上形成的核蛋白细丝在37℃的体外催化RNA转录本与单链DNA序列的杂交。RecA蛋白迅速促进杂交，即使当非互补RNA比杂交RNA多出一千倍的核苷酸，以及比杂交的单链DNA多出一千倍的核苷酸时。异源双链DNA和RecA包被的非互补单链DNA也是体外产生的RNA转录本的劣势竞争对手。由于大量的非互补RNA在温和的非降解条件下不能显著抑制RecA蛋白的杂交反应，该反应可能有助于鉴定和分离体内产生的转录本。
The nucleoprotein filament formed by the RecA protein of Escherichla coll on single-stranded DNA catalyzes the hybridization of RNA transcripts with singlestranded DNA sequences at 37°C, In vitro. RecA protein rapidly promotes hybridization, even when noncomplementary RNA is In a milllonfold nucleotide excess over hybridizing RNA, and in a thousandfold nucleotide excess over hybridizing single-stranded DNA. Heterologous double-stranded DNA and RecAcoated noncomplementary single-stranded DNA are also poor competitors of RNA transcripts producedin vitro. Since large excesses of noncomplementary RNA fail to inhibit sharply the hybridization reaction by RecA protein under mild, non-degradative conditions, the reaction may be useful in the identification and isolation of transcripts produced In vivo.