Differentiation of HPV-containing cells using organotypic "raft" culture or methylcellulose.
Differentiation of HPV-containing cells using organotypic "raft" culture or methylcellulose.
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使用器官型“筏”培养物或甲基纤维素分化含有 HPV 的细胞。
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
L. Laimins
中科院分区：
文献类型：
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作者：
Regina Wilson;L. Laimins
人类乳头瘤病毒(HPV)的研究一直是具有挑战性的，因为其生产生命周期的分化依赖方面。从组织中分离的HPV病毒粒子用于研究病毒的发病机制是复杂的，因为在组织培养中合成的HPV病毒粒子数量很少，而且对细胞的感染效率很低。作为一种替代方法，已开发出遗传学方法来研究乳头瘤病毒在其自然宿主--人角质形成细胞中的生命周期。已经开发出用克隆的HPV DNA转染角质形成细胞和分离保持病毒DNA作为染色体外元件的细胞系的技术。由于HPV生命周期的生产阶段依赖于分化，体外组织培养模型也被用来概括上皮分化。器官型木筏培养中的分化以及在半固体介质中悬浮时的分化都被用来研究病毒生命周期的早期和晚期。
The study of human papillomaviruses (HPVs) has been challenging due to the differentiation-dependent aspects of their productive life cycles. The use of HPV virions, isolated from tissues, to study viral pathogenesis has been complicated due to the low numbers of HPV virions synthesized and inefficient infection of cells in tissue culture. As an alternative approach, genetic methods have been developed to study the papillomavirus life cycle in its natural host, human keratinocytes. Techniques have been developed to transfect keratinocytes with cloned HPV DNA and to isolate cell lines that maintain viral DNA as extra-chromosomal elements. Since the productive phase of the HPV life cycle is dependent on differentiation, in vitro tissue-culture models have also been used to recapitulate epithelial differentiation. Differentiation in organotypic raft cultures as well as upon suspension in semi-solid media have been used to study both early and late stages of the viral life cycle.