The electronic structure of Fe2+ in reaction centers from Rhodopseudomonas sphaeroides. II. Extended x-ray fine structure studies.
The electronic structure of Fe2+ in reaction centers from Rhodopseudomonas sphaeroides. II. Extended x-ray fine structure studies.
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球形红假单胞菌反应中心 Fe2 的电子结构。
DOI：
10.1016/s0006-3495(82)84698-5
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发表时间：
1982
影响因子：
3.4
通讯作者：
Feher,G
中科院分区：
文献类型：
--
作者：
Eisenberger,P;Okamura,MY;Feher,G
利用扩展X射线吸收精细结构（EXAFS）方法对光合细菌Rhodopyrusphaeroides R-26的反应中心进行了研究。研究了含有两个、一个和不含醌（2 Q、1 Q、0 Q）样品的RC。第一配位壳层的平均配体距离被确定为2.10 +/- 0.02 A，更远的壳层为4.14 +/- 0.05 A。发现RC中的Fe 2+位点具有非常大的结构无序参数，由此推断出每个铁位点的配体距离的扩展约为+/-0.1 A。第一个壳层最可能的配位数是6，以氧和氮的混合物作为配体。边吸收结果与Fe ~（2+）处于畸变八面体环境的结果一致。发现含和不含邻菲咯啉的2 Q和1 Q样品的EXAFS谱是相同的。这表明仲醌和邻菲咯啉不与Fe 2+结合，或者它们取代了等价的配体。0 Q样品显示EXAFS振幅降低12%，在添加邻菲咯啉后恢复。这些结果可以解释为一个配体的损失或严重的构象变化时，伯醌被删除。
Extended x-ray absorption fine structure (EXAFS) studies were performed on reaction centers (RC) of the photosynthetic bacterium Rhodopseudomonas sphaeroides R-26. RC containing two, one, and no quinones (2Q, 1Q, 0Q) samples were studied. The average ligand distance of the first coordination shell was determined to be 2.10 +/- 0.02 A with a more distant shell at 4.14 +/- 0.05 A. The Fe2+ site in RC was found to have a very large structural disorder parameter, from which a spread in ligand distance per iron site of approximately +/- 0.1 A was deduced. The most likely coordination number of the first shell is six, with a mixture of oxygens and nitrogens as ligands. The edge absorption results are consistent with the Fe2+ being in distorted octahedral environment. The EXAFS spectra of the 2Q and 1Q samples with and without O-phenanthroline were found to be the same. This indicates that either the secondary quinone and o-phenanthroline do not bind to Fe2+ or that they replace an equivalent ligand. The 0Q sample showed a 12% decrease in the EXAFS amplitude, which was restored upon addition of o-phenanthroline. These results can be explained by either a loss of a ligand or a severe conformational change when the primary quinone was removed.
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金属蛋白的 X 射线吸收光谱。
影响因子：
--
作者：
S. Chan;R. Gamble
通讯作者：
R. Gamble
细胞色素 c 与球形红假单胞菌 R-26 反应中心的相互作用：通过平衡透析测定结合位点数量和解离常数。
影响因子：
2.9
作者：
Rosen,D;Okamura,MY;Feher,G
通讯作者：
Feher,G
细菌光合作用的主要受体：泛醌在球状红假单胞菌光活性反应中心中的必然作用。
DOI：
--
发表时间：
1975
影响因子：
11.1
作者：
Okamura My;R. Isaacson;G. Feher
通讯作者：
G. Feher
影响因子：
56.9
作者：
EISENBERGER, P;KINCAID, BM
通讯作者：
KINCAID, BM
球形红假单胞菌反应中心 Fe2 的电子结构。
影响因子：
3.4
作者：
Butler,WF;Johnston,DC;Shore,HB;Fredkin,DR;Okamura,MY;Feher,G
通讯作者：
Feher,G