Metabolomics study on the effects of Buchang Naoxintong capsules for treating cerebral ischemia in rats using UPLC-Q/TOF-MS
Metabolomics study on the effects of Buchang Naoxintong capsules for treating cerebral ischemia in rats using UPLC-Q/TOF-MS
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UPLC-Q/TOF-MS代谢组学研究步长脑心通胶囊治疗大鼠脑缺血的作用
DOI：
10.1016/j.jep.2016.01.016
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发表时间：
2016-03-02
影响因子：
5.4
通讯作者：
Yang, Hongjun
中科院分区：
文献类型：
--
作者：
Liu, Mengting;Liu, Xin;Yang, Hongjun
民族药理学相关性：步长脑心通胶囊（BNC）在中医中广泛用于治疗脑血管和心血管疾病。然而，治疗效果和机制尚不清楚。 材料和方法：在本研究中，进行了基于 UPLC/TOF-MS 的代谢组学研究，以探索潜在的生物标志物，以增加我们对脑缺血的了解，并评估 BNC 在大脑中动脉闭塞 (MCAO) 大鼠模型中的整体疗效。通过多变量数据分析分析血浆代谢谱并鉴定代谢生物标志物。结果：在假手术组、MCAO 治疗组和 BNC 治疗组之间观察到明显的分离。我们使用预测 (VIP) 值的可变重要性 (VIP > 1) 和 t 检验 (P
Ethnopharmacological relevance: Buchang Naoxintong Capsules (BNC) are widely prescribed in Chinese medicine for the treatment of cerebrovascular and cardiovascular diseases. However, the therapeutic effects and mechanisms are not yet well understood.Materials and methods: In this study, a UPLC/TOF-MS-based metabolomic study was conducted to explore potential biomarkers that will increase our understanding of cerebral ischemia and to assess the integral efficacy of BNC in a middle cerebral artery occlusion (MCAO) rat model. Plasma metabolic profiles were analyzed and metabolic biomarkers were identified through multivariate data analysis.Results: Clear separations were observed between the sham, MCAO and BNC-treated groups. We identified 28 biomarkers in the MCAO rats using variable importance for the projections (VIP) values (VIP > 1) and a t-test (P