Overexpression of Global Regulator PbrlaeA Leads to the Discovery of New Polyketide in Fungus Penicillium Brocae HDN-12-143
Overexpression of Global Regulator PbrlaeA Leads to the Discovery of New Polyketide in Fungus Penicillium Brocae HDN-12-143
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DOI：
10.3389/fchem.2020.00270
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发表时间：
2020-04
影响因子：
5.5
通讯作者：
Lu Wang;Xianyan Zhang;Kaijin Zhang;Xiaoming Zhang;T. Zhu;Qian Che;Guojian Zhang;Dehai Li
中科院分区：
文献类型：
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作者：
Lu Wang;Xianyan Zhang;Kaijin Zhang;Xiaoming Zhang;T. Zhu;Qian Che;Guojian Zhang;Dehai Li
通过对brocae青霉hdd -12-143的PbrlaeA基因的过表达，分离出了4个构型未见报道的化合物，包括熏蒸素氯醇(1)和一个新化合物异熏蒸素氯醇(2)。根据综合光谱数据、13C NMR计算和ECD计算，确定了所有结构，包括绝对构型。化合物1和2对HL-60具有细胞毒活性，IC50分别为18.63和24.83 μM。
Overexpression of the PbrlaeA gene of the fungus Penicillium brocae HDN-12-143 resulted in the isolation of four compounds including fumigatin chlorohydrin (1), whose configuration has not been reported before, and one new compound iso-fumigatin chlorohydrin (2). All structures including absolute configurations were elucidated on the basis of comprehensive spectroscopic data, 13C NMR calculations, and ECD calculations. Compounds 1 and 2 exhibited cytotoxic activity against HL-60 with IC50 of 18.63 and 24.83 μM.