Gene expression screening of human mast cells and eosinophils using high-density oligonucleotide probe arrays: abundant expression of major basic protein in mast cells
Gene expression screening of human mast cells and eosinophils using high-density oligonucleotide probe arrays: abundant expression of major basic protein in mast cells
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DOI：
10.1182/blood.v98.4.1127
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发表时间：
2001-08-15
期刊：
影响因子：
20.3
通讯作者：
Saito, H
中科院分区：
文献类型：
--
作者：
Nakajima, T;Matsumoto, K;Saito, H
肥大细胞（MC）和嗜酸性粒细胞被认为在引发过敏性炎症中发挥重要作用。使用高密度寡核苷酸探针阵列在人类 MC 和嗜酸性粒细胞中的 12000 个基因中筛选细胞类型特异性基因表达。与其他白细胞相比，MC 表达 140 个细胞类型特异性转录本，而嗜酸性粒细胞仅表达 34 个。在预期的 MC 特异性蛋白（如类胰蛋白酶）的转录本中，主要碱性蛋白（MBP）（曾被认为是嗜酸性粒细胞特异性的）在增加的 MC 特异性信使 RNA 数量方面排名第四。成熟的嗜酸性粒细胞几乎缺乏这种转录本。通过使用流式细胞术和共聚焦激光扫描显微镜分析，发现从 4 个不同来源（即肺、皮肤、成人外周血祖细胞和脐带血祖细胞以及嗜酸性粒细胞）获得的 MC 颗粒中含有高水平的 MBP 蛋白。目前发现 MC 可以产生丰富的 MBP 是至关重要的，因为许多关于过敏发病机制的报告都是基于早期的发现，即 MBP 几乎是嗜酸性粒细胞所独有的，而不是由 MC 产生的。
Mast cells(MCs) and eosinophils are thought to play important roles in evoking allergic inflammation. Cell-type-specific gene expression was screened among 12000 genes in human MCs and eosinophils with the use of high-density oligonucleotide probe arrays. In comparison with other leukocytes, MCs expressed 140 cell-type-specific transcripts, whereas eosinophils expressed only 34. Among the transcripts for expected MC-specific proteins such as tryptase, major basic protein (MBP), which had been thought to be eosinophil specific, was ranked fourth in terms of amounts of increased MC-specific messenger RNA. Mature eosinophils were almost lacking this transcript. MCs obtained from 4 different sources (ie, lung, skin, adult peripheral blood progenitor-derived and cord blood progenitor-derived MCs, and eosinophils) were found to have high protein levels of MBP in their granules with the use of flow cytometric and confocal laser scanning microscopic analyses. The present finding that MCs can produce abundant MBP is crucial because many reports regarding allergic pathogenesis have been based on earlier findings that MBP was almost unique to eosinophils and not produced by MCs.