Glucosamine supplementation demonstrates a negative effect on intervertebral disc matrix in an animal model of disc degeneration.
Glucosamine supplementation demonstrates a negative effect on intervertebral disc matrix in an animal model of disc degeneration.
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DOI：
10.1097/brs.0b013e318286b31e
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发表时间：
2013-05-20
期刊：
影响因子：
3
通讯作者：
Sowa G
中科院分区：
文献类型：
--
作者：
Jacobs L;Vo N;Coelho JP;Dong Q;Bechara B;Woods B;Hempen E;Hartman R;Preuss H;Balk J;Kang J;Sowa G
基于实验室的体内对照研究 确定口服氨基葡萄糖硫酸盐对椎间盘退变的体内作用 尽管氨基葡萄糖已证明对关节软骨有益，但临床益处尚不确定。 CDC 2009 年的一份报告称，许多患者正在使用葡萄糖胺补充剂来治疗腰痛 (LBP)，但没有明显的证据支持其使用。由于椎间盘退变是腰痛的主要原因，我们在椎间盘退变的动物模型中探讨了葡萄糖胺对椎间盘基质稳态的影响。十八只骨骼成熟的新西兰白兔分为四组：对照组、环形穿刺组、葡萄糖胺组、环形穿刺+葡萄糖胺组。接受氨基葡萄糖治疗的兔子每天口服 107 毫克补充剂（基于体重相当于人类 1500 毫克/天）。环形穿刺手术涉及用16G针刺穿3个腰椎间盘的纤维环（AF）以诱导退变。在第 0、4、8、12 和 20 周时获得系列 MRI。 20周时收获椎间盘，用于测定糖胺聚糖（GAG）含量、通过RT-PCR测量的相对基因表达以及组织学分析。研究发现，经氨基葡萄糖治疗的动物环形穿刺损伤椎间盘的 MRI 指数和 NP 面积低于未补充氨基葡萄糖的兔子退变椎间盘的 MRI 指数和 NP 面积。与此一致的是，根据组织学和 GAG 含量测定，葡萄糖胺喂养的动物中糖胺聚糖减少。基因表达与对基质的有害影响一致。这些数据表明，通过基因表达、MRI、组织学和总蛋白聚糖测量，体内动物模型中基质的净效应是抗合成代谢的。这引起了人们对这种常用补充剂的担忧，需要未来的研究来确定这些发现的临床相关性。
Laboratory based controlled in vivo study To determine the in vivo effects of oral glucosamine sulfate on intervertebral disc degeneration Although glucosamine has demonstrated beneficial effect in articular cartilage, clinical benefit is uncertain. A CDC report from 2009 reported that many patients are using glucosamine supplementation for low back pain (LBP), without significant evidence to support its use. Because disc degeneration is a major contributor of LBP, we explored the effects of glucosamine on disc matrix homeostasis in an animal model of disc degeneration. Eighteen skeletally mature New Zealand White rabbits were divided into four groups: control, annular puncture, glucosamine, and annular puncture+glucosamine. Glucosamine treated rabbits received daily oral supplementation with 107mg/day (weight based equivalent to human 1500mg/day). Annular puncture surgery involved puncturing the annulus fibrosus (AF) of 3 lumbar discs with a 16G needle to induce degeneration. Serial MRIs were obtained at 0, 4, 8, 12, and 20 weeks. Discs were harvested at 20 weeks for determination of glycosaminoglycan(GAG) content, relative gene expression measured by RT-PCR, and histological analyses. The MRI index and NP area of injured discs of glucosamine treated animals with annular puncture was found to be lower than that of degenerated discs from rabbits not supplemented with glucosamine. Consistent with this, decreased glycosaminoglycan was demonstrated in glucosamine fed animals, as determined by both histological and GAG content. Gene expression was consistent with a detrimental effect on matrix. These data demonstrate that the net effect on matrix in an animal model in vivo, as measured by gene expression, MRI, histology, and total proteoglycan is anti-anabolic. This raises concern over this commonly used supplement, and future research is needed to establish the clinical relevance of these findings.