Roles of the Foxj1 and Inv genes in the left-right determination of internal organs in mice.
Roles of the Foxj1 and Inv genes in the left-right determination of internal organs in mice.
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DOI：
10.1016/j.bbrc.2005.11.097
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发表时间：
2006-01
影响因子：
3.1
通讯作者：
T. Tamakoshi;Tatsuo Itakura;Abhishek Chandra;T. Uezato;Zhi Yang;Xiaodong Xue;Bo Wang;B. Hackett;Tatsuhiko Yokoyama;N. Miura
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文献类型：
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作者：
T. Tamakoshi;Tatsuo Itakura;Abhishek Chandra;T. Uezato;Zhi Yang;Xiaodong Xue;Bo Wang;B. Hackett;Tatsuhiko Yokoyama;N. Miura
在Foxj 1基因敲除小鼠中，一半显示孤位，而另一半显示逆位，这意味着左右轴的随机确定。相比之下，inv突变小鼠显示出内部器官的镜像配置，这意味着左右轴的反转。虽然这两种突变小鼠的结细胞上有初级纤毛，但它们的表型在偏侧性决定上是不同的。因此，我们制作了Foxj 1/inv双突变小鼠，并分析了它们的表型。我们发现Foxj 1/inv双突变小鼠的表型与Foxj 1突变小鼠的表型比inv突变小鼠的表型更相似。我们还发现右肺异构是Foxj 1突变小鼠和Foxj 1/inv双突变小鼠的主要表型，这可能是由于在两侧板中胚层缺乏Pitx 2表达。这些结果表明，随机信号的偏侧性（Foxj 1）是占主导地位的逆转信号的偏侧性（Inv）。
In Foxj1 knockout mice, half show situs solitus while the other half show situs inversus, which means a random determination of the left–right axis. In contrast, the inv mutant mice show a mirror-image configuration of the internal organs, which means a reversal of the left–right axis. Although these two mutant mice have primary cilia on the nodal cells, their phenotypes are different in laterality determination. We thus made Foxj1/inv double mutant mice and analyzed their phenotype. We found the phenotypes of Foxj1/inv double mutant mice to be more similar to those of the Foxj1 mutant mice than those of the inv mutant mice. We also found right pulmonary isomerism to be a major phenotype of the Foxj1 mutant mice and the Foxj1/inv double mutant mice, which is likely due to the absence of the Pitx2 expression at both lateral plate mesoderms. These results indicate that a random signal of laterality (Foxj1) is dominant over the reversal signal of laterality (Inv).