Combined deep-UV induced fluorescence multispectral imaging and resonance-Raman: a dual modality approach for rapid location and identification of in-theatre biological contaminants
Combined deep-UV induced fluorescence multispectral imaging and resonance-Raman: a dual modality approach for rapid location and identification of in-theatre biological contaminants
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深紫外诱导荧光多光谱成像和共振拉曼相结合：用于快速定位和识别手术室内生物污染物的双模态方法
DOI：
10.1117/12.2621607
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Stothard D
中科院分区：
文献类型：
--
作者：
Stothard D
对高空间和物质特异性的双重要求使得表面生物污染物的远距离战区生物检测极具挑战性。我们将描述一种新的组合荧光多光谱成像（MSI）和远离拉曼的方法，这是通过使用深紫外（亚250 nm激发统一。这允许在相机视场上对候选污染位点进行高置信度定位和分类，以及随后对这些识别位点进行共振拉曼分类。通过使用新型、极高通量的空间外差光谱仪实现了远距离拉曼。该方法的可行性通过其在应用相关生物模拟样品上的使用得到证实。
The dual requirement for high spatial and substance specificity makes stand-off in-theatre biological detection of surface biological contaminants extremely challenging. We will describe a novel combined fluorescence multispectral imaging (MSI) and stand-off Raman approach which are united through their use of deep-UV (sub-250 nm excitation. This allows high-confidence location and classification of candidate contamination sites over the camera field of view, and subsequent resonance-Raman classification of these identified sites. Stand-off Raman is enabled through the use of a novel, extremely high-throughput Spatial Heterodyne spectrometer. The viability of this approach is confirmed through its use on application relevant biological simulant samples.