An antibiotic fusaricidin: a cyclic depsipeptide from Paenibacillus polymyxa E681 induces systemic resistance against Phytophthora blight of red-pepper
An antibiotic fusaricidin: a cyclic depsipeptide from Paenibacillus polymyxa E681 induces systemic resistance against Phytophthora blight of red-pepper
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DOI：
10.1007/s12600-012-0263-z
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发表时间：
2013-02-01
期刊：
影响因子：
1.4
通讯作者：
Park, Kyungseok
中科院分区：
文献类型：
--
作者：
Lee, Seo Hyeon;Cho, Young Eun;Park, Kyungseok
本研究从多粘拟芽孢杆菌E681(E681)中分离得到一种环状抗菌肽--褐腐菌素，研究了其对辣椒疫病的防治作用。Fusaricidin对辣椒疫霉的最小抑菌浓度为16ppm。在辣椒植株中，0.1ppm浓度的褐腐菌素处理后，辣椒疫霉的病情严重度比水处理对照(81.7%)高40%，而处理前的病情严重度分别为45%和83.3%。叶面喷洒和土壤浸泡处理对褐腐病有显著的抑制作用(P<；0.05)。在第一次试验中，用1.0ppm的褐腐菌素浸渍土壤后，病情严重度显著降低到3.3%，而水处理对照的病情严重度为83.3%。与阳性对照(43.1%)和水处理对照(66.2%)相比，0.1ppm的Fusaricidin使辣椒疫霉的病情严重程度降低到27.5%。叶面渗入1.0ppm的褐腐菌素对烟草软腐病有抑制作用。RT-PCR分析表明，野生型拟南芥(COL-0)的病程相关(PR)基因表达上调，而PR基因没有积累，这表明其保护机制可能是基于水杨酸依赖的途径。这是Fusaricidin除了作为抗菌剂表现出对植物病原体的保护作用外，还具有保护作用的首次报道。因此，E681可以通过分泌包括褐腐菌素在内的ISR激发子在植物保护中发挥作用。
In this study fusaricidin, a cyclic depsipeptide isolated from Paenibacillus polymyxa E681 (E681), was demonstrated to control Phytophthora blight infection caused by Phytophthora capsici in red-pepper. The minimal inhibitory concentration (MIC) of fusaricidin was found to be 16 ppm against P. capsici. The disease severity of P. capsici was 40% at 0.1 ppm of fusaricidin when compared with water-treated control (81.7%) on post-treatment, whereas the disease severities on pre-treatment were 45% and 83.3% in fusaricidin (0.1 ppm) and water-treated control, respectively, in red-pepper plants. Significant (P < 0.05) disease suppression was observed on treatment with fusaricidin (0.1 ppm) by foliar spray and soil drench. The disease severity was drastically reduced to 3.3% by soil drench of fusaricidin (1.0 ppm), whereas in water-treated control, the disease severity was 83.3% in the first experiment. Fusaricidin at 0.1 ppm reduced disease severity of P. capsici to 27.5% when compared with positive control (43.1%) and water-treated control (66.2%) in the second experiment. Soft rot disease in tobacco was suppressed upon treatment with fusaricidin at 1.0 ppm by leaf infiltration. RT-PCR analyses of Arabidopsis thaliana revealed that there was an up-regulation of pathogenesis-related (PR) gene expression in wild type A. thaliana (Col-0), while there was no accumulation of PR genes, which implies that the mechanism of protection might be based on a salicylic acid-dependent pathway. This is the first report that fusaricidin exhibits protection against plant pathogens in addition to activity as an antibiotic agent. Hence, E681 can play a role in plant protection by secretion of ISR elicitors including fusaricidin.