Inhibition of aflatoxin B1-induced gamma-glutamyltranspeptidase positive (GGT+) hepatic preneoplastic foci and tumors by low protein diets: evidence that altered GGT+ foci indicate neoplastic potential.
Inhibition of aflatoxin B1-induced gamma-glutamyltranspeptidase positive (GGT+) hepatic preneoplastic foci and tumors by low protein diets: evidence that altered GGT+ foci indicate neoplastic potential.
复制标题
低蛋白饮食对黄曲霉毒素 B1 诱导的 γ-谷氨酰转肽酶阳性 (GGT) 肝肿瘤前病灶和肿瘤的抑制：GGT 病灶改变表明肿瘤潜力的证据。
DOI：
10.1093/carcin/13.9.1607
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发表时间：
1992
期刊：
影响因子：
4.7
通讯作者：
Campbell,TC
中科院分区：
文献类型：
--
作者：
Youngman,LD;Campbell,TC
本实验室先前对年幼Fischer 344雄性大鼠进行的研究表明，低蛋白喂养可显著抑制黄曲霉毒素B1（ATB 1）诱导的γ-谷氨酰转肽酶阳性（GGT+）肝病灶的启动后发展，即使能量摄入量更高。然而，这种饮食效应并不一定适用于肝肿瘤的发展。因此，本研究旨在检查这种饮食对肝肿瘤发展的影响，并在此过程中确定病灶发展与肿瘤发展的相关性。在AFB 1给药后（15天，每天一次，每次0.3 mg/kg），动物被喂食含有6%、14%或22%酪蛋白（5.2%、12.2%、19.1%蛋白质）的饲料，持续6、12、40、58和100周。12周时的病灶和40、58和100周时的肿瘤与蛋白质摄入量呈剂量依赖性。病灶的发展，肿瘤的发病率，肿瘤的大小和每只动物的肿瘤数量显着减少，而调肿瘤的出现增加与低蛋白饲料。非肝肿瘤的发病率也较低的动物喂养的最低蛋白质的饮食。病灶发育指数（病灶数量，肝脏体积百分比）与58周和100周的肿瘤发生率高度相关（r= 0.90-1.00）。尽管能量摄入更大，但肿瘤和病灶抑制发生。
Previous studies in this laboratory with young Fischer 344 male rats have shown that the post-initiation development of aflatoxin B1(ATB1)-induced gamma-glutamyltranspeptidase positive (GGT+) hepatic foci was markedly inhibited by low protein feeding, even though the energy intake was greater. This dietary effect, however, did not necessarily apply to hepatic tumor development. Thus, the present investigation was undertaken to examine this dietary effect upon the development of hepatic tumors and, in so doing, to determine the correlation of foci development with tumor development. Following AFB1dosing (15 daily doses of 0.3 mg/kg each), animals were fed diets containing 6, 14 or 22% casern (5.2, 12.2, 19.1% protein) for 6, 12, 40, 58 and 100 weeks. Foci at 12 weeks and tumors at 40, 58 and 100 weeks developed dose-dependently to protein intake. Foci development, tumor incidence, tumor size and the number of tumors per animal were markedly reduced while the tune to tumor emergence was increased with low protein feeding. Non-hepatic tumor incidence also was lower in the animals fed the lowest protein diet. Foci development indices (foci number, per cent liver volume occupied) were highly correlated with tumor incidence at 58 and 100 weeks (r= 0.90–1.00). Tumor and foci inhibition occurred in spite of the greater energy intake.