Cell reservoirs in lymph nodes infected with HIV-1 subtype E differ from subtype B:: identification by combined in situ polymerase chain reaction and immunohistochemistry
Cell reservoirs in lymph nodes infected with HIV-1 subtype E differ from subtype B:: identification by combined in situ polymerase chain reaction and immunohistochemistry
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DOI：
10.1038/modpathol.3800527
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发表时间：
2006-02-01
期刊：
影响因子：
7.5
通讯作者：
Thorner, PS
中科院分区：
文献类型：
--
作者：
Bhoopat, L;Rithaporn, TS;Thorner, PS
在泰国，主要的艾滋病毒亚型是E，而不是北美和欧洲的亚型B。亚型E具有在朗格汉斯细胞中体外复制的能力。我们假设这种细胞类型可能构成了HIV病毒在受感染淋巴结中的储存库。我们检查了25例HIV-1亚型E感染患者的淋巴结，以确定HIV-1感染细胞的免疫表型、数量和分布。通过原位逆转录-聚合酶链反应或免疫过氧化物酶检测HIV的存在。使用基于碱性磷酸酶的免疫组织化学通过双标记确定细胞身份。淋巴结中的大多数HIV感染细胞是朗格汉斯细胞（CD 1a +S100+）和朗格汉斯相关树突状细胞（p55+S100+）。这些细胞位于淋巴结的副皮质区，少数细胞散布在生发中心的边缘，但生发中心本身不存在，与报道的B亚型病毒的分布相反。此外，多核巨细胞在HIV感染的淋巴结（64%）中比对照组（4%）更常见（P = 0.00002）。总之，朗格汉斯组织细胞和相关细胞是淋巴结中HIV亚型E的储存库。阻断郎格罕细胞及相关细胞的感染途径可能是一种有效的干预E亚型HIV传播的策略。
In Thailand, the predominant HIV subtype is E, rather than subtype B as in North America and Europe. Subtype E has the ability to replicate in vitro in Langerhans cells. We hypothesized that this cell type might constitute a reservoir for the HIV virus in infected lymph nodes. We examined lymph nodes from 25 HIV-1 subtype E-infected patients to determine the immunophenotype of HIV-1-infected cells, their numbers and their distribution. The presence of HIV was detected either by in situ reverse transcriptase-polymerase chain reaction or immunoperoxidase. Cell identity was determined by double labelling using alkaline phosphatase-based immunohistochemistry. The majority of HIV-infected cells in the lymph nodes were Langerhans cells (CD1a+S100+) and Langerhans-related dendritic cells (p55+S100+). These cells were located in the paracortical areas of lymph nodes, with a few cells scattered at the edges of germinal centers, but were absent from germinal centers themselves, in contrast to the reported distribution of subtype B virus. In addition, multinucleated giant cells were significantly more common in HIV-infected nodes (64%) compared to controls (4%) ( P = 0.00002). In conclusion, Langerhans histiocytes and related cells are reservoirs for HIV subtype E in lymph nodes. Disrupting the pathway of infection of Langerhans cells and related cells may be a viable strategy to interfere with transmission of HIV subtype E.