Antivirogram or Phenosense: A Comparison of their Reproducibility and an Analysis of their Correlation
Antivirogram or Phenosense: A Comparison of their Reproducibility and an Analysis of their Correlation
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抗病毒图或现象学：其再现性的比较及其相关性分析
DOI：
10.1177/135965350400900501
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发表时间：
2003
影响因子：
1.2
通讯作者：
J. Mittler
中科院分区：
文献类型：
--
作者：
Kai Wang;R. Samudrala;J. Mittler
抗病毒图和表型检测是广泛应用于HIV耐药性的表型检测方法。每种检测方法的可重复性数据有限，对两者之间的相关性知之甚少。利用斯坦福HIV耐药数据库的数据，我们对每项检测的可重复性进行了全面分析，并使用一般IC50倍变化截断值和药物特异性截断值计算了两项检测的相关性和一致性。虽然检测内相关性很高（Antivirogram和PhenoSense检测的等级相关系数分别为r=0.94和r=0.95），但检测间相关性相当低（r=0.36）。分别使用Antivirogram或PhenoSense检测方法分类为耐药的病毒的药物特异性截断值，只有71.4%[95%置信区间（95% CI）： 58.7-82.1%]和57.0% （95% CI: 45.3-68.1%）的样本被其他检测方法分类为耐药。两种检测方法之间的不一致主要是由于这些检测方法对于低阻值的样品之间的相关性极差（对于抗病毒图测量值分别低于或高于2.0的样品，r=0.02和r=0.61）。由于两种检测方法的临界值都相对较低，我们的分析表明，在解释接近耐药性临界值的测量结果时，应该非常小心。
The Antivirogram and PhenoSense assays are widely used phenotypic tests for HIV drug resistance. There are limited data on the reproducibility of each assay, and little is known about the correlation between the two. Using data from the Stanford HIV drug resistance database, we performed a comprehensive analysis of the reproducibility of each assay, and calculated the correlation and concordance of the two assays using both general IC50 fold change cutoff values and drug-specific cutoff values. Although the within-assay correlations were high (rank correlation coefficients r=0.94 and r=0.95 for the Antivirogram and PhenoSense assays, respectively), the between-assay correlation was considerably lower (r=0.36). Using drug-specific cutoff values for viruses classified as resistant by the Antivirogram or PhenoSense assays, respectively, only 71.4% [95% confidence intervals (95% CI): 58.7–82.1%] and 57.0% (95% CI: 45.3–68.1%) of the samples were classified as resistant using the other assay. The poor agreement between the assays was primarily due to the extremely poor correlation between these assays for samples with low resistance values (r=0.02 and r=0.61 for samples with the Antivirogram measurements lower or higher than 2.0, respectively). Since the cutoff values for both assays are relatively low, our analysis suggests that one should be very careful when interpreting measurements that are near the cutoff values for drug resistance.
影响因子：
158.5
作者：
Little, SJ;Holte, S;Richman, DD
通讯作者：
Richman, DD