Global antibody response to Staphylococcus aureus live-cell vaccination.
Global antibody response to Staphylococcus aureus live-cell vaccination.
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DOI：
10.1038/srep24754
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发表时间：
2016-04-22
影响因子：
4.6
通讯作者：
Ohlsen K
中科院分区：
文献类型：
--
作者：
Selle M;Hertlein T;Oesterreich B;Klemm T;Kloppot P;Müller E;Ehricht R;Stentzel S;Bröker BM;Engelmann S;Ohlsen K
病原体金黄色葡萄球菌会引起多种严重疾病，并因其能够迅速对抗生素物质产生耐药性而令人担忧。高耐药金黄色葡萄球菌感染数量的不断增加，加速了寻找替代治疗方案的进程，以缩小抗金黄色葡萄球菌感染方面不断扩大的差距。金黄色葡萄球菌治疗。本研究分析了 Balb/c 小鼠接种亚致死剂量的活金黄色葡萄球菌后的体液免疫反应。使用最近开发的蛋白质阵列测定静脉内和肌肉内接种疫苗的小鼠血清中引发的抗体模式。我们观察到针对多种金黄色葡萄球菌抗原的特异性抗体反应，这种反应在静脉注射后更强。比我疫苗接种。静脉注射而非肌肉注射疫苗可以保护小鼠免受高剂量细菌的肌肉注射攻击感染。疫苗保护与抗 S 的强度相关。金黄色葡萄球菌抗体反应。这项研究通过使用蛋白质微阵列作为有效工具确定了新型候选疫苗，并表明成功的金黄色葡萄球菌疫苗接种依赖于最佳给药途径。
The pathogen Staphylococcus aureus causes a broad range of severe diseases and is feared for its ability to rapidly develop resistance to antibiotic substances. The increasing number of highly resistant S. aureus infections has accelerated the search for alternative treatment options to close the widening gap in anti-S. aureus therapy. This study analyses the humoral immune response to vaccination of Balb/c mice with sublethal doses of live S. aureus. The elicited antibody pattern in the sera of intravenously and intramuscularly vaccinated mice was determined using of a recently developed protein array. We observed a specific antibody response against a broad set of S. aureus antigens which was stronger following i.v. than i.m. vaccination. Intravenous but not intramuscular vaccination protected mice against an intramuscular challenge infection with a high bacterial dose. Vaccine protection was correlated with the strength of the anti-S. aureus antibody response. This study identified novel vaccine candidates by using protein microarrays as an effective tool and showed that successful vaccination against S. aureus relies on the optimal route of administration.