Spatially distinct, temporally stable microbial populations mediate biogeochemical cycling at and below the seafloor in hydrothermal vent fluids
Spatially distinct, temporally stable microbial populations mediate biogeochemical cycling at and below the seafloor in hydrothermal vent fluids
复制标题
DOI：
10.1111/1462-2920.14011
复制
发表时间：
2018-02-01
影响因子：
5.1
通讯作者：
Huber, Julie A.
中科院分区：
文献类型：
--
作者：
Fortunato, Caroline S.;Larson, Benjamin;Huber, Julie A.
在深海热液喷口，微生物群落在海底上方、下方和下方的地球化学梯度上繁衍生息。在这项研究中，我们确定了微生物群落和特定种群的基因内容和转录模式，以了解地球化学上不同的扩散流体热液喷口在空间和时间上的分类和代谢。 2013 年至 2015 年，通过宏基因组学、宏转录组学、基因组分箱和地球化学分析，对轴海山（东北太平洋胡安德富卡海脊上的一座活海底火山）的三个不同喷口（海葵、标记 33 和标记 113）的喷口流体进行了检查。结果表明，随着时间的推移，各个喷口位点维持了微生物群落和特定种群，但 具有空间上不同的分类学、代谢潜力和基因转录谱。每个喷口的地球化学和物理结构在塑造每个地点存在的优势生物和新陈代谢方面都发挥着重要作用。基因组分箱确定了 SUP05、Aquificales 和产甲烷古菌的关键种群，这些种群进行了碳、硫、氢和氮的重要转化，其中的种群对于各个位点来说似乎是独特的。这项工作强调了海底及海底以下微生物代谢过程、流体化学和微生物种群动态之间的联系，并增进了对热液喷口微生物群落在深海生物地球化学循环中作用的了解。
At deep-sea hydrothermal vents, microbial communities thrive across geochemical gradients above, at, and below the seafloor. In this study, we determined the gene content and transcription patterns of microbial communities and specific populations to understand the taxonomy and metabolism both spatially and temporally across geochemically different diffuse fluid hydrothermal vents. Vent fluids were examined via metagenomic, metatranscriptomic, genomic binning, and geochemical analyses from Axial Seamount, an active submarine volcano on the Juan de Fuca Ridge in the NE Pacific Ocean, from 2013 to 2015 at three different vents: Anemone, Marker 33, and Marker 113. Results showed that individual vent sites maintained microbial communities and specific populations over time, but with spatially distinct taxonomic, metabolic potential, and gene transcription profiles. The geochemistry and physical structure of each vent both played important roles in shaping the dominant organisms and metabolisms present at each site. Genomic binning identified key populations of SUP05, Aquificales and methanogenic archaea carrying out important transformations of carbon, sulfur, hydrogen, and nitrogen, with groups that appear unique to individual sites. This work highlights the connection between microbial metabolic processes, fluid chemistry, and microbial population dynamics at and below the seafloor and increases understanding of the role of hydrothermal vent microbial communities in deep ocean biogeochemical cycles.