In contrast to kindled seizures, the frequency of spontaneous epilepsy in the limbic status model correlates with greater aberrant fascia dentata excitatory and inhibitory axon sprouting, and increased staining for N-methyl-D-aspartate, AMPA and GABA(A) receptors
In contrast to kindled seizures, the frequency of spontaneous epilepsy in the limbic status model correlates with greater aberrant fascia dentata excitatory and inhibitory axon sprouting, and increased staining for N-methyl-D-aspartate, AMPA and GABA(A) receptors
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DOI：
10.1016/s0306-4522(96)00516-7
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发表时间：
1997-04-01
期刊：
影响因子：
3.3
通讯作者：
Mendoza, D
中科院分区：
文献类型：
--
作者：
Mathern, GW;Bertram, EH;Mendoza, D
本研究通过比较边缘癫痫持续状态后自发性癫痫大鼠和点燃发作频率相近的动物，确定海马神经元丢失和突触可塑性是否存在差异。在弗吉尼亚大学，SD大鼠被植入双侧海马腹侧电极，处理如下：无刺激(电极对照；n=5)：无状态刺激(刺激对照；n=5)；持续刺激边缘状态(n=12)。对边缘状态组进行至少四周的脑电监测。四只大鼠没有记录的慢性癫痫发作(状态对照组)，所有三个对照组在海马区病理上没有差异，因此被归入一个组(对照组)。8只边缘状态动物最终发展为慢性癫痫(自发性癫痫发作)，另外8只大鼠点燃的5级癫痫发作次数和频率与自发性癫痫发作组相似。在加州大学(UCLA)，对海马区进行了如下处理：(I)尼氏染色检测神经元密度；(Ii)neo-Timm‘s组织化学染色检测杂乱纤维发芽；(Iii)免疫细胞化学染色检测谷氨酸脱羧酶、N-甲基-D-天冬氨酸受体亚单位2、AMPA受体亚单位1和GABA(A)受体。用计算机图像分析技术对齿状筋膜内、外分子层的neo-Timm‘s染色和免疫反应以灰度值进行量化。经统计学处理，差异有统计学意义(P
This study determined whether there were differences in hippocampal neuron loss and synaptic plasticity by comparing rats with spontaneous epilepsy after limbic status epilepticus and animals with a similar frequency of kindled seizures. At the University of Virginia, Sprague-Dawley rats were implanted with bilateral ventral hippocampal electrodes and treated as follows; no stimulation (electrode controls; n=5): hippocampal stimulation without status (stimulation controls; n=5); and limbic status from continuous hippocampal stimulation (n=12). The limbic status group were electrographically monitored for a minimum of four weeks. Four rats had no recorded chronic seizures (status controls), and all three control groups showed no differences in hippocampal pathology and were therefore incorporated into a single group (controls). Eight limbic status animals eventually developed chronic epilepsy (spontaneous seizures) and an additional eight rats were kindled to a similar number and frequency of stage 5 seizures (kindled) as the spontaneous seizures group. At the University of California (UCLA) the hippocampi were processed for: (i) Nissl stain for densitometric neuron counts; (ii) neo-Timm's histochemistry for messy fiber sprouting; and (iii) immunocytochemical staining for glutamate decarboxylase, N-methyl-D-aspartate receptor subunit 2, AMPA receptor subunit 1 and the GABA(A) receptor. In the fascia dentata inner and outer molecular layers the neo-Timm's stain and immunoreactivity was quantified as gray values using computer image analysis techniques. Statistically significant results (P