Diversity of apostome ciliates, Chromidina spp. (Oligohymenophorea, Opalinopsidae), parasites of cephalopods of the Mediterranean Sea.
Diversity of apostome ciliates, Chromidina spp. (Oligohymenophorea, Opalinopsidae), parasites of cephalopods of the Mediterranean Sea.
复制标题
DOI：
10.1051/parasite/2016033
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Grellier P
中科院分区：
文献类型：
--
作者：
Souidenne D;Florent I;Dellinger M;Justine JL;Romdhane MS;Furuya H;Grellier P
染色藻属是一种神秘的使徒纤毛虫（寡水足纲，Opalinopsidae），寄生在头足类动物的肾脏和胰腺附属物上。只有四个物种被描述，其中只有三个被正式命名。到目前为止还没有DNA序列的报道。目的研究染色丝藻属（Chromidina spp.）多样性，我们采样头足类动物在地中海突尼斯，突尼斯，并确定了两个不同的Chromidina种。在两种不同的宿主物种中：普通枪乌贼和乌贼。从苏木精染色的幻灯片，我们描述了这些寄生虫种的形态特征，并将它们与以前的描述进行比较。我们还根据Chatton和Lwoff的原始材料重新描述了优雅铬藻（Chromidina elegans，Foettinger，1881）的形态，并将其命名为新单体型和副新单体型。本文描述了一新种，Chattoni Chromidina souvonne，Florent and Grelier n.例如，在L。vulgaris在突尼斯外海，以及一个可能的新物种的证据，在S. officinalis在突尼斯近海，尽管后一种与以前描述的Chromidina cortezi Hochberg，1971的一些形态阶段相似。我们扩增，为第一次，这两个Chromidina物种的18S rDNA标记。系统发育分析支持Chromidina在apostome纤毛虫中的关联。代表性的apostomes的18S rDNA序列之间的遗传距离分析表明，Pseudocollinia是最接近的属Chromidina。
Chromidina spp. are enigmatic apostome ciliates (Oligohymenophorea, Opalinopsidae) that parasitise the renal and pancreatic appendages of cephalopods. Only four species have been described, among which only three have been formally named. No DNA sequence has been reported so far. To investigate Chromidina spp. diversity, we sampled cephalopods in the Mediterranean Sea off Tunis, Tunisia, and identified two distinct Chromidina spp. in two different host species: Loligo vulgaris and Sepia officinalis. From haematoxylin-stained slides, we described morphological traits for these parasitic species and compared them to previous descriptions. We also re-described the morphology of Chromidina elegans (Foettinger, 1881) from Chatton and Lwoff’s original materials and designated a neohapantotype and paraneohapantotypes for this species. We describe a new species, Chromidina chattoni Souidenne, Florent and Grellier n. sp., found in L. vulgaris off Tunisia, and evidence for a probable novel species, found in S. officinalis off Tunisia, although this latter species presents similarities to some morphological stages previously described for Chromidina cortezi Hochberg, 1971. We amplified, for the first time, an 18S rDNA marker for these two Chromidina species. Phylogenetic analysis supports the association of Chromidina within apostome ciliates. Genetic distance analysis between 18S rDNA sequences of representative apostomes indicates Pseudocollinia as the most closely related genus to Chromidina.