Competence and commitment of Caenorhabditis elegans vulval precursor cells.
Competence and commitment of Caenorhabditis elegans vulval precursor cells.
复制标题
秀丽隐杆线虫外阴前体细胞的能力和承诺。
DOI：
10.1006/dbio.1999.9357
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Sternberg,PW
中科院分区：
文献类型：
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作者：
Wang,M;Sternberg,PW
多能性隐杆线虫外阴前体细胞（VPCs）在三种命运（1°、2°和3°）中选择响应两种细胞间信号：EGF家族生长因子LIN-3诱导高水平的1°命运和低水平的2°命运；通过受体LIN-12的信号诱导2°命运。如果LIN-3信号的水平由于LIN-3的亚态突变而降低，那么最靠近锚细胞（AC）的VPC的子细胞P6。通过在LIN-3缺乏的动物中表达LIN-3作为时间的函数，我们发现VPC及其子代都能对LIN-3做出反应，而VPC子代在与皮下组织融合后失去了反应能力。我们还证明了指定为2°的vpc的子节点可以响应LIN-3，这表明2°的vpc不是不可逆的。我们建议在第一个细胞周期后维持VPC能力和1°命运的优先级有助于确保P6。P变成1°。这种能力调节机制可能从祖先线虫物种中得以维持，它们在VPC分裂之前和之后都使用了诱导，从而最大限度地提高了形成功能性外阴的可能性。
Multipotent Caenorhabditis elegans vulval precursor cells (VPCs) choose among three fates (1°, 2°, and 3°) in response to two intercellular signals: the EGF family growth factor LIN-3 induces 1° fates at high levels and 2° fates at low levels; and a signal via the receptor LIN-12 induces 2° fates. If the level of LIN-3 signal is reduced by a lin-3 hypomorphic mutation, the daughters of the VPC closest to the anchor cell (AC), P6.p, are induced by the AC. By expressing LIN-3 as a function of time in LIN-3-deficient animals, we find that both VPCs and the daughters of VPCs are competent to respond to LIN-3, and VPC daughters lose competence after fusing with the hypodermis. We also demonstrate that the daughters of VPCs specified to be 2° can respond to LIN-3, indicating that 2° VPCs are not irreversibly committed. We propose that maintenance of VPC competence after the first cell cycle and the prioritization of the 1° fate help ensure that P6.p will become 1°. This mechanism of competence regulation might have been maintained from ancestral nematode species that used induction both before and after VPC division and serves to maximize the probability that a functional vulva is formed.