Slippery Liquid-Infused Porous Surfaces that Prevent Bacterial Surface Fouling and Inhibit Virulence Phenotypes in Surrounding Planktonic Cells.
Slippery Liquid-Infused Porous Surfaces that Prevent Bacterial Surface Fouling and Inhibit Virulence Phenotypes in Surrounding Planktonic Cells.
复制标题
DOI：
10.1021/acsinfecdis.6b00065
复制
发表时间：
2016-07-08
影响因子：
5.3
通讯作者：
Lynn, David M.
中科院分区：
文献类型：
--
作者：
Kratochvil, Michael J.;Welsh, Michael A.;Manna, Uttam;Ortiz, Benjamin J.;Blackwell, Helen E.;Lynn, David M.
表面既可以防止细菌生物污染，又可以抑制周围浮游细菌的毒力表型表达，这在广泛的背景下引起了人们的兴趣。在这里，我们报道了新的光滑液体注入的多孔表面（SLIPS），它可以抵抗细菌定植（由于固有的“光滑”表面特性），并通过逐渐释放小分子群体感应抑制剂（qsi）来减弱非贴壁细胞的毒力表型。对铜绿假单胞菌有活性的qsi可以加载到slip中，而不会失去其防滑和防污性能，并且根据负载剂的结构，嵌入剂可以在数小时至数天内释放到周围介质中。这种控释方法对抑制毒力因子的产生很有用，也可以抑制细菌生物膜在附近的非slip涂层表面的形成。最后，我们证明了这种方法与同时释放多种类型的QSI兼容，从而更好地控制毒性，并为调整这些光滑表面的防污性能提供了新的机会。
Surfaces that can both prevent bacterial biofouling and inhibit the expression of virulence phenotypes in surrounding planktonic bacteria are of interest in a broad range of contexts. Here, we report new slippery-liquid infused porous surfaces (SLIPS) that resist bacterial colonization (owing to inherent ‘slippery’ surface character) and also attenuate virulence phenotypes in non-adherent cells by gradually releasing small-molecule quorum sensing inhibitors (QSIs). QSIs active against Pseudomonas aeruginosa can be loaded into SLIPS without loss of their slippery and anti-fouling properties, and imbedded agents can be released into surrounding media over hours to days depending on the structures of the loaded agent. This controlled-release approach is useful for inhibiting virulence factor production and can also inhibit bacterial biofilm formation on nearby, non-SLIPS-coated surfaces. Finally, we demonstrate that this approach is compatible with the simultaneous release of more than one type of QSI, enabling greater control over virulence and suggesting new opportunities to tune the anti-fouling properties of these slippery surfaces.
登录
查看更多内容
DOI：
10.1039/c0cc02316g
发表时间：
2011-01-07
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Breitbach AS;Broderick AH;Jewell CM;Gunasekaran S;Lin Q;Lynn DM;Blackwell HE
通讯作者：
Blackwell HE
影响因子：
3.2
作者：
Glansdorp, FG;Thomas, GL;Spring, DR
通讯作者：
Spring, DR
影响因子：
4.9
作者：
Gomes, Jose;Grunau, Alexander;Gademann, Karl
通讯作者：
Gademann, Karl
影响因子：
14
作者：
Ho, Kitty K. K.;Chen, Renxun;Kumar, Naresh
通讯作者：
Kumar, Naresh
影响因子：
10
作者：
Broderick, Adam H.;Stacy, Danielle M.;Lynn, David M.
通讯作者：
Lynn, David M.