Cysteines 153 and 154 of Transmembrane Transforming Growth Factor-α Are Palmitoylated and Mediate Cytoplasmic Protein Association*
Cysteines 153 and 154 of Transmembrane Transforming Growth Factor-α Are Palmitoylated and Mediate Cytoplasmic Protein Association*
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跨膜转化生长因子-α 的半胱氨酸 153 和 154 被棕榈酰化并介导细胞质蛋白关联*
DOI：
--
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发表时间：
1996
影响因子：
4.8
通讯作者：
R. Derynck
中科院分区：
文献类型：
--
作者：
L. Shum;C. Turck;R. Derynck
Transforming growth factor-α (TGF-α) is synthesized as a transmembrane protein with a highly conserved, short cytoplasmic domain that is rich in cysteines. TGF-α is a prototype of a large family of growth factors involved in cell-cell communication. We have shown previously that transmembrane TGF-α associates with a kinase activity and two proteins of 106 and 86 kDa. In this study, we have used site-directed mutagenesis of the cytoplasmic domain of TGF-α to define the structural requirements for these protein interactions. Whereas the cytoplasmic domain of TGF-α was not essential for association with transmembrane p106, deletion of the C-terminal 8 amino acids, including a cysteine pair, abolished the interaction with p86 and greatly reduced the kinase activity associated with transmembrane TGF-α. Replacement of these 2 cysteines by serines similarly reduced the association of p86 with transmembrane TGF-α. Using a combination of mutational analysis and direct microsequencing, we have determined that this cysteine pair was palmitoylated. We therefore conclude that these cysteines play a critical role in the interaction of TGF-α with associated proteins and in the function of this protein complex. The palmitoylation of these cysteines suggests a possibly dynamic role of fatty acid modification in the integrity and function of the transmembrane TGF-α complex.
DOI：
10.1016/s0021-9258(18)52927-6
发表时间：
1993-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
L. Paige;M. Nadler;M. Harrison;J. Cassady;R. Geahlen
通讯作者：
L. Paige;M. Nadler;M. Harrison;J. Cassady;R. Geahlen
蛋白质的脂肪酰化。
DOI：
10.1146/annurev.cb.04.110188.003143
发表时间：
1988
期刊：
Annual review of cell biology
影响因子：
--
作者：
Schultz,AM;Henderson,LE;Oroszlan,S
通讯作者：
Oroszlan,S
主要组织相容性复合体抗原跨膜区的半胱氨酸通过硫酯键被脂肪酰化。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kaufman,JF;Krangel,MS;Strominger,JL
通讯作者：
Strominger,JL