A novel strategy for the preparation of liposomes: rapid solvent exchange
A novel strategy for the preparation of liposomes: rapid solvent exchange
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DOI：
10.1016/s0005-2736(99)00006-1
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发表时间：
1999-03-04
影响因子：
3.4
通讯作者：
Feigenson, GW
中科院分区：
文献类型：
--
作者：
Buboltz, JT;Feigenson, GW
在多组分模型膜的制备过程中，主要考虑的是组成均匀性应在整个悬浮液中占主导地位。一些常规的样品制备方法使脂质混合物通过中间的无溶剂状态。这是一种有序的固态，并且可能有利于膜组分的分层。一种新的制备方法已经开发出来，这是专门设计来避免这种中间状态。这种新的策略被称为快速溶剂交换（RSE），需要在有机溶剂和水性缓冲液之间直接转移脂质混合物。RSE脂质体需要不超过一分钟的时间来制备和表现出相当大的包封体积，具有高比例的外表面积。在高胆固醇含量的磷脂/胆固醇混合物中，通过更常规的方法制备的悬浮液显示人为分层的证据，而通过快速溶剂交换制备的样品则没有。讨论了在常规样品制备过程中可能导致人为分层的原理。(C)1999 Elsevier Science B. V.保留所有权利。
During the preparation of multi-component model membranes, a primary consideration is that compositional homogeneity should prevail throughout the suspension. Some conventional sample preparation methods pass the lipid mixture through an intermediary, solvent-free state. This is an ordered, solid state and may favor the demixing of membrane components. A new preparative method has been developed which is specifically designed to avoid this intermediary state. This novel strategy is called rapid solvent exchange (RSE) and entails the direct transfer of lipid mixtures between organic solvent and aqueous buffer. RSE liposomes require no more than a minute to prepare and manifest considerable entrapment volumes with a high fraction of external surface area. In phospholipid/cholesterol mixtures of high cholesterol content, suspensions prepared by more conventional methods reveal evidence of artifactual demixing, whereas samples prepared by rapid solvent exchange do not. The principles which may lead to artifactual demixing during conventional sample preparation are discussed. (C) 1999 Elsevier Science B.V. All rights reserved.