Chloroquine increases Plasmodium falciparum gametocytogenesis in vitro
Chloroquine increases Plasmodium falciparum gametocytogenesis in vitro
复制标题
DOI：
10.1017/s0031182099003960
复制
发表时间：
1999-04-01
期刊：
影响因子：
2.4
通讯作者：
Read, AF
中科院分区：
文献类型：
--
作者：
Buckling, A;Ranford-Cartwright, LC;Read, AF
疟疾寄生虫能够调节将资源从无性生长转移到蚊子(配子体)感染阶段的产生。配子体生成率的增加似乎是对压力的普遍反应，既是自然遇到的，也是新奇的。我们之前已经报道了在体内，在啮齿动物疟疾中，查鲍迪疟原虫对亚治疗抗疟疾化疗的反应发生了更早和更多的配子细胞生成。使用免疫荧光法检测侵入红细胞单层的寄生虫，我们证明了在体外，人类疟疾恶性疟原虫的配子体生成增加了5倍，这是对抗疟疾药物氯喹的治疗的反应。在所使用的所有克隆中，配子体生成率随着氯喹对无性生长抑制程度的增加而增加。此外，在胁迫和配子体产生之间的关系上存在克隆差异，这意味着这种反应是遗传可变的。然而，这与氯喹耐药性无关。对这些结果的流行病学意义进行了讨论。
Malaria parasites are capable of modulating the diversion of resources from asexual growth to the production of stages infective to mosquitoes (gametocytes). Increased rates of gametocytogenesis appear to be a general response to stress, both naturally encountered and novel. We have previously reported earlier and greater gametocytogenesis in response to subcurative antimalarial chemotherapy in the rodent malaria, Plasmodium chabaudi, in vivo. Using an immunofluorescent assay to detect parasites that had invaded red blood cell monolayers, we demonstrate a 5-fold increase in gametocytogenesis in the human malaria, P. falciparum, in vitro, in response to treatment with the antimalarial drug chloroquine. In all clones used, gametocytogenesis increased with increasing inhibition of asexual growth by chloroquine. Furthermore, there were clone differences in the relationship between stress and gametocyte production, implying the response was genetically variable. This was not, however, associated with chloroquine resistance. The epidemiological significance of these results is discussed.