Differentiation of diastereomeric N-acetylhexosamine monosaccharides using ion trap tandem mass spectrometry
Differentiation of diastereomeric N-acetylhexosamine monosaccharides using ion trap tandem mass spectrometry
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DOI：
10.1021/ac981052y
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发表时间：
1999-05-15
影响因子：
7.4
通讯作者：
Leary, JA
中科院分区：
文献类型：
--
作者：
Desaire, H;Leary, JA
四极杆离子阱质谱仪配备电喷雾电离用于区分三个非对映体单糖，N-乙酰葡糖胺，N-乙酰半乳糖胺，N-乙酰甘露糖胺。这些化合物被衍生化形成金属络合物[Co-III（DAP）（2）HexNAc]Cl-3，当碰撞活化时，其产生显著不同的产物离子光谱。然后使用三种单糖非对映异构体生成的产物离子光谱来确认水解寡糖中N-乙酰己糖胺的立体化学。最后，通过同位素标记研究确定了每个产物离子的来源，并提出了解释每个产生的解离的机制。
A quadrupole ion trap mass spectrometer equipped with electrospray ionization was used to distinguish three diastereomeric monosaccharides, N-acetylglucosamine, N-acetylgalactosamine, and N-acetylmannosamine. The saccharides were derivatized to form the metal complex [Co-III(DAP)(2)HexNAc]Cl-3 which, when collisionally activated, produced dramatically different product ion spectra. The product ion spectra generated for the three monosaccharide diastereomers were then used to confirm the stereochemistry of N-acetylhexosamines from a hydrolyzed oligosaccharide. Finally, the origin of each product ion was determined through isotopic labeling studies, and mechanisms were proposed which explain each resulting dissociation.