AID in aging and autoimmune diseases.
AID in aging and autoimmune diseases.
复制标题
DOI：
10.3109/08916934.2012.750300
复制
发表时间：
2013-03
期刊：
影响因子：
3.5
通讯作者：
Blomberg BB
中科院分区：
文献类型：
--
作者：
Frasca D;Andrisani G;Diaz A;Felice C;Guidi L;Blomberg BB
本研究的目的是评价接种了2009年大流行（p）流感疫苗的炎症性肠病（IBD）患者和不同年龄的健康个体的B细胞应答的质量。通过血凝抑制（HAI）试验测量体内反应，该试验代表了与疫苗保护性最确定的相关性。通过在疫苗刺激的PBMC培养物中活化诱导的胞苷脱氨酶（AID）来测量体外应答。在接受抗TNF-α治疗的IBD患者中，这两种应答均受到一定程度的损害，但在接受抗TNF-α和免疫抑制（IS）药物治疗的IBD患者中，这两种应答降低得更多。后者患者体内和体外B细胞反应与老年人相似。此外，正如我们先前在健康受试者中所证明的，对多克隆刺激CpG的体外应答可用作后续疫苗应答的生物标志物，并且AID活化与IBD患者中的血清应答相关，就像在健康个体中一样。这些结果共同表明，与接受单药治疗的IBD患者相比，接受抗TNF-α和IS治疗的IBD患者体内和体外B细胞应答显著受损。这是第一份报告，以证明B细胞缺陷，如测量的自主AID报告，在IBD患者有助于减少体液反应的流感疫苗，因为我们以前已经显示了老年人。
The aim of this study was to evaluate the quality of B cell responses in patients with Inflammatory Bowel Disease (IBD) and healthy individuals of different ages, vaccinated with the pandemic (p)2009 influenza vaccine. The in vivo response was measured by the hemagglutination inhibition (HAI) assay, which represents the most established correlate with vaccine protectiveness. The in vitro response was measured by activation-induced cytidine deaminase (AID) in cultures of vaccine-stimulated PBMC. Both responses are somewhat impaired in IBD patients undergoing anti-TNF-α treatment but these are much more decreased in IBD patients undergoing treatment with anti-TNF-α and immunosuppressive (IS) drugs. These latter patients had in vivo and in vitro B cell responses similar to those of elderly individuals. Moreover, as we have previously demonstrated in healthy subjects, the in vitro response to the polyclonal stimulus CpG may be used as a biomarker for subsequent vaccine response and AID activation is correlated with the serum response in IBD patients, as it is in healthy individuals. These results altogether indicate that IBD patients on anti-TNF-α and IS have significantly impaired in vivo and in vitro B cell responses, as compared to those on monotherapy. This is the first report to demonstrate that B cell defects, as measured by the autonomous AID reporter, in IBD patients contribute to reduced humoral responses to the influenza vaccine, as we have previously shown for elderly individuals.