Reef-associated crustacean fauna: biodiversity estimates using semi-quantitative sampling and DNA barcoding
Reef-associated crustacean fauna: biodiversity estimates using semi-quantitative sampling and DNA barcoding
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DOI：
10.1007/s00338-009-0543-3
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发表时间：
2009-12-01
期刊：
影响因子：
3.5
通讯作者：
Meyer, C.
中科院分区：
文献类型：
--
作者：
Plaisance, L.;Knowlton, N.;Meyer, C.
与珊瑚礁有关的隐生物群占这些生态系统生物多样性的主要部分，但在生物多样性估计中基本上被忽视，因为这些生物很难收集和识别。我们结合联合收割机的半定量抽样设计和DNA条形码的方法，提供指标的珊瑚礁相关的甲壳动物的多样性。22个类似大小的死头Pocilloppora采样10米深的五个太平洋中部地区（四个环礁在北方线群岛和莫雷阿，法属波利尼西亚）。所有的甲壳类动物被删除，部分细胞色素氧化酶亚基I从403个人测序，产生135个不同的类群使用物种水平的标准5%的相似性。大多数甲壳类物种是罕见的; 44%的OTU由单个个体代表，另外33%由仅在五个地点之一发现的几个标本代表。北方线群岛和莫雷阿岛只有11个OTU。物种丰富度统计（Chao 1和ACE）估计的总数表明，至少有90种甲壳类动物在莫雷阿岛和150在北方线岛屿的这种栖息地类型。然而，每个区域的稀疏曲线未能接近渐近线，并且Chao 1和ACE估计量在Moorea中采样8个头后没有稳定，因此即使这些多样性数字也被低估了。然而，即使是这种从非常有限的栖息地进行的适度采样工作，也导致了惊人的高物种数量。
The cryptofauna associated with coral reefs accounts for a major part of the biodiversity in these ecosystems but has been largely overlooked in biodiversity estimates because the organisms are hard to collect and identify. We combine a semi-quantitative sampling design and a DNA barcoding approach to provide metrics for the diversity of reef-associated crustacean. Twenty-two similar-sized dead heads of Pocillopora were sampled at 10 m depth from five central Pacific Ocean localities (four atolls in the Northern Line Islands and in Moorea, French Polynesia). All crustaceans were removed, and partial cytochrome oxidase subunit I was sequenced from 403 individuals, yielding 135 distinct taxa using a species-level criterion of 5% similarity. Most crustacean species were rare; 44% of the OTUs were represented by a single individual, and an additional 33% were represented by several specimens found only in one of the five localities. The Northern Line Islands and Moorea shared only 11 OTUs. Total numbers estimated by species richness statistics (Chao1 and ACE) suggest at least 90 species of crustaceans in Moorea and 150 in the Northern Line Islands for this habitat type. However, rarefaction curves for each region failed to approach an asymptote, and Chao1 and ACE estimators did not stabilize after sampling eight heads in Moorea, so even these diversity figures are underestimates. Nevertheless, even this modest sampling effort from a very limited habitat resulted in surprisingly high species numbers.