S-Click Reaction for Isotropic Orientation of Oxidases on Electrodes to Promote Electron Transfer at Low Potentials.
S-Click Reaction for Isotropic Orientation of Oxidases on Electrodes to Promote Electron Transfer at Low Potentials.
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DOI：
10.1002/anie.201909203
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发表时间：
2019-10
影响因子：
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通讯作者：
Lin Xia;Ming-jie Han;Lu Zhou;Aiping Huang;Zhaoya Yang;Tianyuan Wang;Fa-hui Li;Lu Yu;C. Tian;Zhongsheng Zang;Qing‐Zheng Yang;Chenli Liu;Wenxu Hong;Yi Lu;L. Alfonta;Jiangyun Wang
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文献类型：
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作者：
Lin Xia;Ming-jie Han;Lu Zhou;Aiping Huang;Zhaoya Yang;Tianyuan Wang;Fa-hui Li;Lu Yu;C. Tian;Zhongsheng Zang;Qing‐Zheng Yang;Chenli Liu;Wenxu Hong;Yi Lu;L. Alfonta;Jiangyun Wang
电化学传感器对于即时检测（POCT）和可穿戴传感设备至关重要。在氧化还原酶和电极之间建立有效的电子传递途径是将酶催化反应转化为电化学信号的关键，也是开发稳定、灵敏和选择性生物传感器的关键。我们证明，一种新的合成氨基酸（2-氨基-3-（4-巯基苯基）丙酸）的位点特异性掺入到氧化还原酶，然后通过S-点击反应将酶连接到电极，促进电子转移。制造的生物传感器表现出实时和选择性监测血液和汗液样品中的色氨酸（Trp），线性范围为0.02-0.8 mm。沿着这条路线的进一步发展沿着可能会导致便携式电化学传感器的急剧扩张，为不同的健康决定分子。
Electrochemical sensors are essential for point-of-care testing (POCT) and wearable sensing devices. Establishing an efficient electron transfer route between redox enzymes and electrodes is key for converting enzyme-catalyzed reactions into electrochemical signals, and for the development of robust, sensitive, and selective biosensors. We demonstrate that the site-specific incorporation of a novel synthetic amino acid (2-amino-3-(4-mercaptophenyl)propanoic acid) into redox enzymes, followed by an S-click reaction to wire the enzyme to the electrode, facilitates electron transfer. The fabricated biosensor demonstrated real-time and selective monitoring of tryptophan (Trp) in blood and sweat samples, with a linear range of 0.02-0.8 mm. Further developments along this route may result in dramatic expansion of portable electrochemical sensors for diverse health-determination molecules.