Dual crosslinked chondroitin sulfate injectable hydrogel formed via continuous Diels-Alder (DA) click chemistry for bone repair
Dual crosslinked chondroitin sulfate injectable hydrogel formed via continuous Diels-Alder (DA) click chemistry for bone repair
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通过连续 Diels-Alder (DA) 点击化学形成的双交联硫酸软骨素可注射水凝胶用于骨修复
DOI：
10.1016/j.carbpol.2017.02.062
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发表时间：
2017-06-15
影响因子：
11.2
通讯作者：
Liu, Mingzhu
中科院分区：
文献类型：
--
作者：
Bai, Xiao;Lu, Shaoyu;Liu, Mingzhu
在本工作中，通过DA点击化学在F127-AMI（马来酰亚胺封端的F127）和ChS-呋喃（糠胺接枝的硫酸软骨素）之间合成了在37 ℃下具有低凝胶浓度的热敏共聚物F127@ChS（F127交联的硫酸软骨素）。然后，基于F127@ChS和PEG-AMI（马来酰亚胺封端的聚乙二醇）制备双重交联水凝胶。F127@ChS的物理交联提供了水凝胶的快速凝胶化行为，而F127@ChS与PEG-AMI之间发生的原位DA点击反应提供了水凝胶体系的共价交联。将该双交联可注射水凝胶作为骨形态发生蛋白-4（BMP-4）的支架材料用于大鼠颅骨缺损的修复。术后12周X线片、头颅数字图像及组织学（HE和Masson染色）观察显示，缺损区有新生骨组织形成。结果表明，新型的双重交联可注射水凝胶为颅骨组织工程提供了一个有趣的选择。(C)2017爱思唯尔有限公司版权所有
In the present work, a thermosensetive copolymer with a low gelation concentration under 37 degrees C, F127@ChS (F127 crosslinked chondroitin sulfate) was synthesized via DA click chemistry between F127-AMI (maleimido terminated F127) and ChS-furan (furfurylamine grafted chondroitin sulfate). Then, dual crosslinked hydrogels were prepared based on F127@ChS and PEG-AMI (maleimido terminated polyethylene glycol). The physical crosslinking of F127@ChS affords the hydrogel fast gelation behavior, while in situ DA click reaction occurred between F127@ChS and PEG-AMI affords the hydrogel system covalent crosslinking. The dual crosslinked injectable hydrogel was applied as scaffold to load BMP-4 for rat cranial defect repair. As indicated by X-ray imaging, cranial digital images and histological (HE and Masson) staining analysis, new bone tissues were formed in the defected area after 12 weeks repair. The results demonstrate that the novel dual crosslinked injectable hydrogel offer an interesting option for cranial bone tissue engineering. (C) 2017 Elsevier Ltd. All rights reserved.