Identification of new tumor suppressor genes based on in vivo functional inactivation of a candidate gene
Identification of new tumor suppressor genes based on in vivo functional inactivation of a candidate gene
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DOI：
10.1016/s0014-5793(99)00598-0
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发表时间：
1999-05-28
期刊：
影响因子：
3.5
通讯作者：
Zabarovsky, ER
中科院分区：
文献类型：
--
作者：
Li, JF;Protopopov, AI;Zabarovsky, ER
作为开发用于鉴定肿瘤抑制基因的新功能测试的一步，使用两种新载体：pLNCtTA和pETI，在四环素阻遏物的转录调节下，在小鼠A9纤维肉瘤细胞系中仅表达人野生型和突变RE基因。在免疫缺陷SCID小鼠中转染子传代后，野生型RE基因被删除或功能丧失 在所有 20 个测试的肿瘤中第一次传代后就已经失活。相比之下，在所有 10 个研究的肿瘤中都保留了一个无功能的突变 RE 基因。这些结果表明，肿瘤抑制基因的鉴定测试可能基于它们在体内的功能失活，而不是基于生长抑制。 (C) 1999 年欧洲生化学会联合会。
As a step towards developing a new functional test for the identification of tumor suppressor genes, human wild type and mutant RE genes mere expressed in the mouse A9 fibrosarcoma cell line under the transcriptional regulation of the tetracycline repressor using two new vectors: pLNCtTA and pETI, Following passage of the transfectants in immunodeficient SCID mice, the wild type RE gene,vas deleted or functionally inactivated already after the first passage in all 20 tumors tested. In contrast, a non-functional mutant RE gene,vas maintained in all 10 tumors studied. These results suggest that tests for the identification of tumor suppressor genes may be based on their functional inactivation in vivo, rather than on growth suppression. (C) 1999 Federation of European Biochemical Societies.