Usefulness of D2-40 immunohistochemistry for differentiation between kaposiform hemangioendothelioma and tufted angioma
Usefulness of D2-40 immunohistochemistry for differentiation between kaposiform hemangioendothelioma and tufted angioma
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DOI：
10.1111/j.1600-0560.2006.00461.x
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发表时间：
2006-07-01
影响因子：
1.7
通讯作者：
Shimizu, Michio
中科院分区：
文献类型：
--
作者：
Arai, Eiichi;Kuramochi, Akira;Shimizu, Michio
背景：最近的研究已经证明了单克隆抗体D2-40作为淋巴内皮的标记物的效用。D2-40可用于福尔马林固定材料和石蜡包埋材料。我们的目的是利用D2-40免疫组化技术阐明毛细血管瘤之间的差异，特别是卡样血管内皮瘤（KHE）和簇状血管瘤（TA）之间的差异。我们研究了4例KHE， 9例TA和31例其他血管肿瘤。同时应用CD31、CD34、viii因子相关抗原和GLUT1抗体。结果：KHE中，2-40在卡波西肉瘤样增生性毛细血管周围有3例明显反应，4例中有1例有部分反应，周围扩张血管呈阴性。TA时，周围扩张血管D2-40部分阳性，炮弹样增生毛细血管D2-40部分阴性。结论：D2-40是KHE和TA免疫组化鉴别的有效抗体。此外，D2-40免疫染色模式的差异仅限于毛细血管增生周围和周围扩张血管；因此，我们认为KHE和TA可能反映了单一实体演化的不同阶段。也就是说，它们可能起源于具有淋巴细胞和血管内皮细胞血统特征的干细胞。
Background:Recent investigations have demonstrated the utility of the monoclonal antibody D2-40 as a marker for lymphatic endothelium. D2-40 can be used on formalin-fixed and paraffin-embedded materials. Our objective was to elucidate, using D2-40 immunohistochemistry, the differences among capillary hemangiomas, and especially between kaposiform hemangioendothelioma (KHE) and tufted angioma (TA). We studied four cases of KHE, nine cases of TA, and 31 cases of other vascular tumors. Antibodies against CD31, CD34, factor VIII-related antigen, and GLUT1 were also applied.Results:In KHE, D2-40 was markedly reactive for three and partially for one of four cases in the peripheral area of Kaposi's sarcoma-like proliferative capillaries and negative in the surrounding dilated vessels. In TA, D2-40 was partially positive in the surrounding dilated vessels and negative in cannonball-like proliferative capillaries.Conclusions:Our results suggest that D2-40 is a useful antibody for immunohistochemical discrimination between KHE and TA. In addition, the difference of immunostaining pattern of D2-40 is limited to the peripheral area of capillary proliferation and surrounding dilated vessels; therefore, it is suggested that KHE and TA may reflect different stages in the evolution of a single entity. Namely, they may originate from stem cells possessing the characteristics of both lymphatic and blood vessel endothelial lineages.