Microfluidic platform to evaluate migration of cells from patients with DYT1 dystonia.
Microfluidic platform to evaluate migration of cells from patients with DYT1 dystonia.
复制标题
用于评估 DYT1 肌张力障碍患者细胞迁移的微流体平台。
DOI：
10.1016/j.jneumeth.2014.05.027
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发表时间：
2014
影响因子：
3
通讯作者：
Irimia,Daniel
中科院分区：
文献类型：
--
作者：
Nery,FlaviaC;Atai,NadiaA;daHora,CintiaC;Kim,EdwardY;Hettich,Jasmin;Mempel,ThorstenR;Breakefield,XandraO;Irimia,Daniel
背景技术用于定量评价细胞生物学过程的微流体平台允许生物学和病理学事件的低成本和时间有效的研究，例如通过实时成像监测细胞迁移。在健康和疾病状态下，细胞迁移对发育和伤口愈合以及维持身体的稳态至关重要。新方法微流控室允许精确测量，以调查是否成纤维细胞携带TOR 1A基因突变，遗传性神经疾病-DYT 1肌张力障碍，结果我们观察到来自DYT 1患者的成纤维细胞在细胞迁移的基本特征上显示出异常，如运动的速度降低和持续性。与现有方法的比较微流控方法使我们能够证明与对照细胞相比，在运动的DYT 1患者细胞内细胞核的极化降低以及细胞核和高尔基体的异常取向，结论我们在这里报告了不同的检测方法，可用于确定DYT 1患者细胞迁移的各种参数细胞作为theTOR1A基因突变的结果，包括一个微流控平台，它提供了一种手段，以评估在三维环境中的单细胞分辨率的实时矢量运动。
BackgroundMicrofluidic platforms for quantitative evaluation of cell biologic processes allow low cost and time efficient research studies of biological and pathological events, such as monitoring cell migration by real-time imaging. In healthy and disease states, cell migration is crucial in development and wound healing, as well as to maintain the body's homeostasis.New methodThe microfluidic chambers allow precise measurements to investigate whether fibroblasts carrying a mutation in theTOR1Agene, underlying the hereditary neurologic disease – DYT1 dystonia, have decreased migration properties when compared to control cells.ResultsWe observed that fibroblasts from DYT1 patients showed abnormalities in basic features of cell migration, such as reduced velocity and persistence of movement.Comparison with existing methodThe microfluidic method enabled us to demonstrate reduced polarization of the nucleus and abnormal orientation of nuclei and Golgi inside the moving DYT1 patient cells compared to control cells, as well as vectorial movement of single cells.ConclusionWe report here different assays useful in determining various parameters of cell migration in DYT1 patient cells as a consequence of theTOR1Agene mutation, including a microfluidic platform, which provides a means to evaluate real-time vectorial movement with single cell resolution in a three-dimensional environment.