Peptidomics-based approach reveals the secretion of the 29-residue COOH-terminal fragment of the putative tumor suppressor protein DMBT1 from pancreatic adenocarcinoma cell lines.
Peptidomics-based approach reveals the secretion of the 29-residue COOH-terminal fragment of the putative tumor suppressor protein DMBT1 from pancreatic adenocarcinoma cell lines.
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DOI：
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发表时间：
2002-09
期刊：
影响因子：
11.2
通讯作者：
K. Sasaki;Kae Sato;Y. Akiyama;K. Yanagihara;M. Oka;K. Yamaguchi
中科院分区：
文献类型：
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作者：
K. Sasaki;Kae Sato;Y. Akiyama;K. Yanagihara;M. Oka;K. Yamaguchi
恶性脑肿瘤抑制蛋白1是脑、肺、食管、胃和结肠直肠癌中的假定肿瘤抑制蛋白。在这里，我们报告的质谱鉴定的3335 Da肽，这是发现在无血清条件培养基从5的15胰腺癌细胞系，但不是从35癌细胞系和2个非恶性胰腺导管细胞系。该肽是恶性脑肿瘤中缺失的29个COOH末端氨基酸1。提示该肽在细胞内通过有限的蛋白水解产生并分泌到细胞外。我们基于肽的方法将有助于筛选特定类型癌症的候选标记肽。
Deleted in malignant brain tumors 1 is a putative tumor suppressor protein in brain, lung, esophageal,gastric, and colorectal cancer. Here we report the mass spectrometric identification of a 3335 Da peptide, which was found in serum-free conditioned medium from 5 of 15 pancreatic adenocarcinoma cell lines but not from 35 carcinoma cell lines and 2 nonmalignant pancreatic duct cell lines. The peptide was the 29 COOH-terminal amino acids from deleted in malignant brain tumors 1. It is suggested that the peptide is generated inside the cells by limited proteolysis and extracellularly secreted. Our peptidomics-based approach will help screen candidate marker peptides for a particular type of cancer.