A RAPID PARTITION ASSAY FOR ROUTINE ANALYSIS OF JUVENILE-HORMONE RELEASE BY INSECT CORPORA ALLATA
A RAPID PARTITION ASSAY FOR ROUTINE ANALYSIS OF JUVENILE-HORMONE RELEASE BY INSECT CORPORA ALLATA
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DOI：
10.1016/0003-2697(81)90575-3
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发表时间：
1981-01-01
影响因子：
2.9
通讯作者：
TOBE, SS
中科院分区：
文献类型：
--
作者：
FEYEREISEN, R;TOBE, SS
描述了一种分配测定法，其允许快速测定昆虫[Diploptera punctata]咽侧体释放保幼激素。将新合成和释放的甲基标记的C16保幼激素定量提取到异辛烷相中;前体甲基标记的L-甲硫氨酸保留在孵育培养基中。当与适当的空白试验结合使用时，该方法提供了咽侧体活性常规分析所需的准确度和速度。
A partition assay is described which allows a rapid determination of juvenile hormone release by insect [Diploptera punctata] corpora allata. Newly synthesized and released methyl-labeled C16 juvenile hormone is quantitatively extracted into an isooctane phase; precursor methyl-labeled L-methionine remains in the incubation medium. When used in conjunction with appropriate blank assays, this method offers both accuracy and speed necessary for routine analysis of corpora allata activity.