MicroRNA profiles in Sorghum exposed to individual drought or heat or their combination
MicroRNA profiles in Sorghum exposed to individual drought or heat or their combination
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DOI：
10.1007/s13562-021-00747-0
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发表时间：
2021-11-26
影响因子：
1.9
通讯作者：
Sunkar，Ramanjulu
中科院分区：
文献类型：
--
作者：
Puli，Chandra Obul Reddy;Zheng，Yun;Sunkar，Ramanjulu
在干旱或高温或其同时发生的半干旱地区，高粱主要用于食品、饲料和生物燃料生产。植物 microRNA (miRNA) 是基因调控网络不可或缺的一部分，该网络控制着几乎所有生物过程，包括适应胁迫条件。迄今为止，已经报道了单独干旱或热胁迫下的植物 miRNA 谱，但尚未报道干旱和热胁迫联合作用下的植物 miRNA 谱。在这项研究中，我们报告了暴露于单独干旱或高温或其组合的高粱叶片中的 miRNA 谱。已鉴定出由 80 个单独 miRNA 代表的约 29 个保守 miRNA 家族、由 47 个不太保守或高粱特异性 miRNA 家族成员代表的 26 个家族以及 8 个新 miRNA 家族。其中，25 个 miRNA 被发现在应激治疗反应中受到差异调节。比较分析表明，与单独干旱相比，热或热和干旱组合下的 miRNA 调节更强。此外，使用降解组测序，48 个基因被确认为高粱中 miRNA 的靶标。总的来说，这项研究为理解组合压力下 miRNA 引导的基因调控提供了一个框架。
Sorghum is largely grown for food, fodder and for biofuel production in semi-arid regions where the drought or high temperature or their combination co-occur. Plant microRNAs (miRNAs) are integral to the gene regulatory networks that control almost all biological processes including adaptation to stress conditions. Thus far, plant miRNA profiles under separate drought or heat stresses have been reported but not under combined drought and heat. In this study, we report miRNA profiles in leaves of sorghum exposed to individual drought or heat or their combination. Approximately 29 conserved miRNA families represented by 80 individual miRNAs, 26 families represented by 47 members of less conserved or sorghum-specific miRNA families as well as 8 novel miRNA families have been identified. Of these, 25 miRNAs were found to be differentially regulated in response to stress treatments. The comparative profiling revealed that the miRNA regulation was stronger under heat or combination of heat and drought compared to the drought alone. Furthermore, using degradome sequencing, 48 genes were confirmed as targets for the miRNAs in sorghum. Overall, this study provides a framework for understanding of the miRNA-guided gene regulations under combined stresses.