Co-linearity of β-Galactosidase with Its Gene by Immunological Detection of Incomplete Polypeptide Chains
Co-linearity of β-Galactosidase with Its Gene by Immunological Detection of Incomplete Polypeptide Chains
复制标题
通过不完整多肽链的免疫学检测β-半乳糖苷酶与其基因的共线性
作者：
A. Fowler;I. Zabin
当在琼脂上针对由天然 β-半乳糖苷酶制备的抗血清进行双重扩散测试时，映射到 β-半乳糖苷酶结构基因中的许多无义突变体产生形成沉淀素系的材料。通过蔗糖密度梯度离心测量的交叉反应物质的相对大小与从无义突变体的遗传图谱计算的大小相同。还指出了蛋白质相对于其基因的方向。
Many nonsense mutants that map in the β-galactosidase structural gene produce material that forms precipitin lines when tested by double diffusion on agar against antiserum prepared from native β-galactosidase. Relative sizes of the cross-reacting materia!s measured by sucrose density gradient centrifugation are the same as sizes calculated from genetic mapping of nonsense mutants. Orientation of the protein to its gene is also indicated.