Basic fibroblast growth factor-mediated lymphangiogenesis of lymphatic endothelial cells isolated from dog thoracic ducts: effects of heparin.
Basic fibroblast growth factor-mediated lymphangiogenesis of lymphatic endothelial cells isolated from dog thoracic ducts: effects of heparin.
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DOI：
10.2170/jjphysiol.48.133
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发表时间：
1998-04
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影响因子：
--
通讯作者：
Y. Tan
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文献类型：
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作者：
Y. Tan
我们试图评价从犬胸导管分离的淋巴管内皮细胞(LEC)在碱性成纤维细胞生长因子(BFGF)刺激下是否具有诱导淋巴管生成的能力，并观察肝素对碱性成纤维细胞生长因子介导的形态发生的影响。通过改变亚融合细胞的数量和伤口移行实验，观察bFGF和/或肝素对LEC增殖和迁移的影响。用相差显微镜和电子显微镜观察药物对晶状体上皮细胞三维胶原凝胶的侵袭和成管以及胶原凝胶诱导的晶状体上皮细胞成管的影响。碱性成纤维细胞生长因子(10 ng/ml)可显著诱导晶状体上皮细胞的增殖和迁移，1-100微克/毫升的肝素可剂量依赖性地促进其增殖和迁移，并使其侵袭和形成三维胶原凝胶。碱性成纤维细胞生长因子还有助于在两层胶原凝胶之间形成毛细血管样的LEC管。肝素(10微克/毫升)可加速碱性成纤维细胞生长因子介导的淋巴管生成。这些结果表明，培养的犬胸导管LEC具有形成毛细淋巴管样管的能力，肝素可剂量依赖性地加速bFGF介导的淋巴管新生血管的形成过程。
We have attempted to evaluate whether, similar to the angiogenesis of blood vessels, cultures of lymphatic endothelial cells (LEC) isolated from dog thoracic ducts have an ability to induce lymphangiogenesis in response to basic fibroblast growth factor (bFGF), then to examine the effects of heparin on the bFGF-mediated morphogenesis. The effects of bFGF and/or heparin on the proliferation and migration of the LEC were evaluated by changing the number of the subconfluent cells and by wound migration assay, respectively. The effects of the agents on invasion and tube formation of the LEC into a three-dimensional collagen gel and on collagen gel induced tube formation of the LEC were also investigated by a phase-contrast microscope and an electron microscope. The bFGF (10 ng/ml) caused a significant induction of proliferation and migration of the LEC, the induction of which was augmented dose-dependently by an additional treatment with heparin ranging from 1 to 100 microg/ml. The bFGF produced invasion and tube formation of the LEC into a three-dimensional collagen gel. The bFGF also facilitated to form capillary-like tubes of the LEC between two layers of collagen gels. Heparin (10 microg/ml) accelerated both processes of bFGF-mediated lymphangiogenesis of the LEC. These findings suggest that the cultured LEC isolated from dog thoracic ducts have an ability to form lymphatic capillary-like tubes in response to bFGF and that heparin accelerates dose-dependently the process of the bFGF-mediated neovascularization of lymph vessels.