Sympathetic nervous system mediates cold stress-induced suppression of natural killer cytotoxicity in rats
Sympathetic nervous system mediates cold stress-induced suppression of natural killer cytotoxicity in rats
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DOI：
10.1016/j.neulet.2003.11.075
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发表时间：
2004-02-26
影响因子：
2.5
通讯作者：
Hisamitsu, T
中科院分区：
文献类型：
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作者：
Jiang, XH;Guo, SY;Hisamitsu, T
本研究旨在探讨6-羟基多巴胺（6-OHDA）作为化学交感神经切断剂抑制冷应激引起的脾自然杀伤细胞（NK）杀伤活性的机制。用（51）铬释放法测定NK活性。中枢交感神经切除术伴侧脑室（i. c. v.）注射6-OHDA可明显降低4 ℃冷应激4 h引起的血浆皮质酮水平升高、下丘脑室旁核和蓝斑Fos表达以及NK活性抑制。外周交感神经切除术，腹膜内（i. p.）注射6-OHDA和腹腔注射普萘洛尔阻断P-肾上腺素能受体也能逆转冷应激对NK细胞毒活性的抑制作用，但对脑内Fos表达无明显影响。结果提示，冷应激诱导的下丘脑-垂体-肾上腺轴激活可能至少部分由中枢去甲肾上腺素能系统介导，冷应激诱导的NK细胞毒活性抑制可能由外周交感神经激活介导。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
The aim of the present study is to investigate the mechanisms of suppression of splenic natural killer (NK) cytotoxicity caused by cold stress, using 6-hydroxydopamine (6-OHDA) as chemical sympathectomy. The NK activity was measured by (51)chromium release assay. Central sympathectomy with intracerebroventricular (i.c.v.) injection of 6-OHDA reduced significantly the elevation of plasma corticosterone level, the expression of Fos in hypothalamic paraventricular nucleus and in locus coeruleus, as well as the suppression of NK activity induced by cold stress at 4 degreesC for 4 h. Peripheral sympathectomy with intraperitoneal (i.p.) injection of 6-OHDA and blockade of P-adrenergic receptor with i.p. injection of propranolol also reversed the cold stress-induced suppression of NK cytotoxicity, but without significant effect on Fos expression in brain. The results suggest that the activation of hypothalamic-pituitary-adrenal axis induced by cold stress might be mediated, at least partially, by central noradrenergic system, and that the cold stress-induced suppression of NK cytotoxicity might be mediated by the activation of peripheral sympathetic nerve. (C) 2003 Elsevier Ireland Ltd. All rights reserved.